Entrapment of β-galactosidase in polyvinylalcohol hydrogel

被引：47
作者：
Grosova, Zuzana [1 ]
Rosenberg, Michal [1 ]
Rebros, Martin [1 ]
Sipocz, Matej [1 ]
Sedlackova, Barbora [1 ]
机构：
[1] Slovak Univ Technol Bratislava, Fac Chem & Food Technol, Inst Biotechnol & Food Sci, Bratislava 81237, Slovakia
来源：
BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 04期
关键词：
beta-galactosidase; hydrolysis; immobilization; lactose; LentiKats; PVA gel;
D O I：
10.1007/s10529-007-9606-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
beta-Galactosidase isolated from Aspergillus oryzae was immobilized in lens-shaped polyvinylalcohol capsules (with activity 25 U g(-1)) giving 32% of its original activity. Immobilization did not change the pH optimum (4.5) of lactose hydrolysis. The relative enzyme activity during product inhibition testing was, in average, 10% higher for immobilized enzyme. No decrease of activity was observed after 35 repeated batch runs and during 530 h of continuous hydrolysis of lactose (10%, w/v) at 45 degrees C. The immobilized enzyme was stable for 14 months without any change of activity during the storage at 4 degrees C and pH 4.5.
引用
收藏
页码：763 / 767
页数：5
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