The C2H2-ZF transcription factor Zfp335 recognizes two consensus motifs using separate zinc finger arrays

被引：20

作者：

Han, Brenda Yuyuan ^{[1
,2
,4
]}

Foo, Chuan-Sheng ^{[3
]}

Wu, Shuang ^{[1
,2
]}

Cyster, Jason G. ^{[1
,2
]}

机构：

[1] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94143 USA

[2] Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94143 USA

[3] Stanford Univ, Dept Comp Sci, Stanford, CA 94305 USA

[4] Agcy Sci Technol & Res, Inst Mol & Cell Biol, Singapore 138632, Singapore

来源：

GENES & DEVELOPMENT | 2016年 / 30卷 / 13期

基金：

美国国家卫生研究院;

关键词：

transcription factors; zinc fingers; protein-DNA binding; Zfp335; ZNF335; DNA-BINDING; GENE; EVOLUTION; SEQUENCE;

D O I：

10.1101/gad.279406.116

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The complexities of DNA recognition by transcription factors (TFs) with multiple Cys2-His2 zinc fingers (C2H2-ZFs) remain poorly studied. We previously reported a mutation (R1092W) in the C2H2-ZF TF Zfp335 that led to selective loss of binding at a subset of targets, although the basis for this effect was unclear. We show that Zfp335 binds DNA and drives transcription via recognition of two distinct consensus motifs by separate ZF clusters and identify the specific motif interaction disrupted by R1092W. Our work presents Zfp335 as a model for understanding how C2H2-ZF TFs may use multiple recognition motifs to control gene expression.

引用

页码：1509 / 1514

页数：6

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