Plasmodium falciparum histidine-rich-protein (PfHRP2 and 3) diversity in Western and Coastal Kenya

被引：25

作者：

Nderu, David ^{[1
,2
]}

Kimani, Francis ^{[3
]}

Thiong'o, Kelvin ^{[3
]}

Karanja, Evaline ^{[4
]}

Akinyi, Maureen ^{[3
]}

Too, Edwin ^{[3
]}

Chege, William ^{[3
]}

Nambati, Eva ^{[3
]}

Meyer, Christian G. ^{[1
,5
,6
]}

Velavan, Thirumalaisamy P. ^{[1
,5
,6
,7
]}

机构：

[1] Univ Tubingen, Inst Trop Med, Tubingen, Germany

[2] Kirinyaga Univ, Sch Hlth Sci, Kerugoya, Kenya

[3] Kenya Govt Med Res Ctr, Ctr Biotechnol Res & Dev, Nairobi, Kenya

[4] Tech Univ Kenya, Sch Biol & Life Sci, Dept Biochem & Biotechnol, Nairobi, Kenya

[5] Vietnamese German Ctr Med Res VG CARE, Hanoi, Vietnam

[6] Duy Tan Univ, Fac Med, Da Nang, Vietnam

[7] Fdn Congolaise Rech Med, Brazzaville, Rep Congo

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

RAPID DIAGNOSTIC-TESTS; GENETIC DIVERSITY; SEQUENCE VARIATION; MALARIA PARASITES; PERFORMANCE; IDENTIFICATION; SENSITIVITY; SENEGAL; IMPACT; VIVAX;

D O I：

10.1038/s41598-018-38175-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Plasmodium falciparum histidine-rich proteins 2 (PfHRP2) based RDTs are advocated in falciparum malaria-endemic regions, particularly when quality microscopy is not available. However, diversity and any deletion in the pfhrp2 and pfhrp3 genes can affect the performance of PfHRP2-based RDTs. A total of 400 samples collected from uncomplicated malaria cases from Kenya were investigated for the amino acid repeat profiles in exon 2 of pfhrp2 and pfhrp3 genes. In addition, PfHRP2 levels were measured in 96 individuals with uncomplicated malaria. We observed a unique distribution pattern of amino acid repeats both in the PfHRP2 and PfHRP3. 228 PfHRP2 and 124 PfHRP3 different amino acid sequences were identified. Of this, 214 (94%) PfHRP2 and 81 (65%) PfHRP3 amino acid sequences occurred only once. Thirty-nine new PfHRP2 and 20 new PfHRP3 amino acid repeat types were identified. PfHRP2 levels were not correlated with parasitemia or the number of PfHRP2 repeat types. This study shows the variability of PfHRP2, PfHRP3 and PfHRP2 concentration among uncomplicated malaria cases. These findings will be useful to understand the performance of PfHRP2-based RDTs in Kenya.

引用

页数：9

共 43 条

[1]

[Anonymous], 2016, CCBY-NC-SA 3.0 IGO

[2]

[Anonymous], 2018, Depression

[3]

[Anonymous], 2016, FALS NEG RDT RES IMP

[4] Genetic variation of pfhrp2 in Plasmodium falciparum isolates from Yemen and the performance of HRP2-based malaria rapid diagnostic test [J].

Atroosh, Wahib M. ;

Al-Mekhlafi, Hesham M. ;

Al-Jasari, Adel ;

Sady, Hany ;

Al-Delaimy, Ahmed K. ;

Nasr, Nabil A. ;

Dawaki, Salwa ;

Abdulsalam, Awatif M. ;

Ithoi, Init ;

Lau, Yee Ling ;

Fong, Mun Yik ;

Surin, Johari .

PARASITES & VECTORS, 2015, 8

[5] Genetic diversity of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and its effect on the performance of PfHRP2-based rapid diagnostic tests [J].

Baker, J ;

McCarthy, J ;

Gatton, M ;

Kyle, DE ;

Belizario, V ;

Luchavez, J ;

Bell, D ;

Cheng, Q .

JOURNAL OF INFECTIOUS DISEASES, 2005, 192 (05) :870-877

[6] Transcription and Expression of Plasmodium falciparum Histidine-Rich Proteins in Different Stages and Strains: Implications for Rapid Diagnostic Tests [J].

Baker, Joanne ;

Gatton, Michelle L. ;

Peters, Jennifer ;

Ho, Mei-Fong ;

McCarthy, James S. ;

Cheng, Qin .

PLOS ONE, 2011, 6 (07)

[7] Research Global sequence variation in the histidine-rich proteins 2 and 3 of Plasmodium falciparum: implications for the performance of malaria rapid diagnostic tests [J].

Baker, Joanne ;

Ho, Mei-Fong ;

Pelecanos, Anita ;

Gatton, Michelle ;

Chen, Nanhua ;

Abdullah, Salim ;

Albertini, Audrey ;

Ariey, Frederic ;

Barnwell, John ;

Bell, David ;

Cunningham, Jane ;

Djalle, Djibrine ;

Echeverry, Diego F. ;

Gamboa, Dionicia ;

Hii, Jeffery ;

Kyaw, Myat Phone ;

Luchavez, Jennifer ;

Membi, Christopher ;

Menard, Didier ;

Murillo, Claribel ;

Nhem, Sina ;

Ogutu, Bernhards ;

Onyor, Pamela ;

Oyibo, Wellington ;

Wang, Shan Qing ;

McCarthy, James ;

Cheng, Qin .

MALARIA JOURNAL, 2010, 9

[8] Rapid diagnostic tests failing to detect Plasmodium falciparum infections in Eritrea: an investigation of reported false negative RDT results [J].

Berhane, Araia ;

Russom, Mulugeta ;

Bahta, Iyassu ;

Hagos, Filmon ;

Ghirmai, Michael ;

Uqubay, Selam .

MALARIA JOURNAL, 2017, 16

[9] Plasmodium falciparum parasites with histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in two endemic regions of Kenya [J].

Beshir, Khalid B. ;

Sepulveda, Nuno ;

Bharmal, Jameel ;

Robinson, Ailie ;

Mwanguzi, Julian ;

Busula, Annette Obukosia ;

de Boer, Jetske Gudrun ;

Sutherland, Colin ;

Cunningham, Jane ;

Hopkins, Heidi .

SCIENTIFIC REPORTS, 2017, 7

[10] Sequence variation in Plasmodium falciparum Histidine Rich Proteins 2 and 3 in Indian isolates: Implications for Malaria Rapid Diagnostic Test Performance [J].

Bharti, Praveen Kumar ;

Chandel, Himanshu Singh ;

Krishna, Sri ;

Nema, Shrikant ;

Ahmad, Amreen ;

Udhayakumar, Venkatachalam ;

Singh, Neeru .

SCIENTIFIC REPORTS, 2017, 7

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