Effect of Biogenic Silver Nanoparticles on the Quorum-Sensing System of Pseudomonas aeruginosa PAO1 and PA14

被引:18
作者
Saeki, Erika Kushikawa [1 ]
Martins, Heloisa Moreira [2 ]
de Camargo, Larissa Ciappina [2 ]
Anversa, Lais [3 ]
Tavares, Eliandro Reis [4 ]
Yamada-Ogatta, Sueli Fumie [4 ]
Yamauchi Lioni, Lucy Megumi [4 ]
Takayama Kobayashi, Renata Katsuko [2 ]
Nakazato, Gerson [2 ]
机构
[1] Adolfo Lutz Inst, Reg Lab Ctr, BR-19013050 Presidente Prudente, SP, Brazil
[2] Univ Estadual Londrina, Biol Sci Ctr, Dept Microbiol, Lab Basic & Appl Bacteriol, BR-86057970 Londrina, Parana, Brazil
[3] Adolfo Lutz Inst, Reg Lab Ctr, BR-17015110 Bauru, SP, Brazil
[4] Univ Estadual Londrina, Biol Sci Ctr, Dept Microbiol, Lab Mol Biol Microorganisms, BR-86057970 Londrina, Parana, Brazil
关键词
AgNPs; antivirulence; gene regulation; quorum quenching; BIOFILM FORMATION; VIRULENCE FACTORS; PATHOGENICITY; INHIBITION; BACTERIA; STRESS;
D O I
10.3390/microorganisms10091755
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The increase in multidrug-resistant microorganisms represents a global threat requiring the development novel strategies to fight bacterial infection. This study aimed to assess the effect of silver nanoparticles (bio-AgNPs) on bacterial growth, biofilm formation, production of virulence factors, and expression of genes related to the quorum-sensing (QS) system of P. aeruginosa PAO1 and PA14. Biofilm formation and virulence assays were performed with bio-AgNPs. RT-qPCR was carried out to determine the effect of bio-AgNPs on the QS regulatory genes lasI, lasR, rhlI, rhlR, pqsA, and mvfR. Bio-AgNPs had an MIC value of 62.50 mu M, for both strains. Phenotypic and genotypic assays were carried out using sub-MIC values. Experimental results showed that treatment with sub-MICs of bio-AgNPs reduced (p < 0.05) the motility and rhamnolipids and elastase production in P. aeruginosa PAO1. In PA14, bio-AgNPs stimulated swarming and twitching motilities as well as biofilm formation and elastase and pyocyanin production. Bio-AgNP treatment increased (p < 0.05) the expression of QS genes in PAO1 and PA14. Despite the different phenotypic behaviors in both strains, both showed an increase in the expression of QS genes. Demonstrating that the bio-AgNPs acted in the induction of regulation. The possible mechanism underlying the action of bio-AgNPs involves the induction of the rhl and/or pqs system of PAO1 and of the las and/or pqs system of PA14. These results suggest that exposure to low concentrations of bio-AgNPs may promote the expression of QS regulatory genes in P. aeruginosa, consequently inducing the production of virulence factors such as elastase, pyocyanin, and biofilms.
引用
收藏
页数:15
相关论文
共 61 条
  • [1] Inhibition of quorum sensing-associated virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1 by Mycoleptodiscus indicus PUTY1
    Ahmed, Tanveer
    Pattnaik, Subhaswaraj
    Khan, Mohd Babu
    Ampasala, Dinakara Rao
    Busi, Siddhardha
    Sarma, V. Venkateswara
    [J]. BRAZILIAN JOURNAL OF MICROBIOLOGY, 2020, 51 (02) : 467 - 487
  • [2] Inhibition of quorum sensing-controlled virulence factors in Pseudomonas aeruginosa by human serum paraoxonase
    Aybey, Aynur
    Demirkan, Elif
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2016, 65 : 105 - 113
  • [3] Attenuation of Pseudomonas aeruginosa quorum sensing, virulence and biofilm formation by extracts of Andrographis paniculata
    Banerjee, Malabika
    Moulick, Soumitra
    Bhattacharya, Kunal Kumar
    Parai, Debaprasad
    Chattopadhyay, Subrata
    Mukherjee, Samir Kumar
    [J]. MICROBIAL PATHOGENESIS, 2017, 113 : 85 - 93
  • [4] Biofilm and Quorum sensing mediated pathogenicity in Pseudomonas aeruginosa
    Brindhadevi, Kathirvel
    LewisOscar, Felix
    Mylonakis, Eleftherios
    Shanmugam, Sabarathinam
    Verma, Tikendra Nath
    Pugazhendhi, Arivalagan
    [J]. PROCESS BIOCHEMISTRY, 2020, 96 (49-57) : 49 - 57
  • [5] Role of quorum sensing in bacterial infections
    Castillo-Juarez, Israel
    Maeda, Toshinari
    Ayerim Mandujano-Tinoco, Edna
    Tomas, Maria
    Perez-Eretza, Berenice
    Julieta Garcia-Contreras, Silvia
    Wood, Thomas K.
    Garcia-Contreras, Rodolfo
    [J]. WORLD JOURNAL OF CLINICAL CASES, 2015, 3 (07) : 575 - 598
  • [6] Revisiting the virulence hallmarks of Pseudomonas aeruginosa: a chronicle through the perspective of quorum sensing
    Chadha, Jatin
    Harjai, Kusum
    Chhibber, Sanjay
    [J]. ENVIRONMENTAL MICROBIOLOGY, 2022, 24 (06) : 2630 - 2656
  • [7] Surface association sensitizes Pseudomonas aeruginosa to quorum sensing
    Chuang, Sara K.
    Vrla, Geoffrey D.
    Froehlich, Kathrin S.
    Gitai, Zemer
    [J]. NATURE COMMUNICATIONS, 2019, 10 (1)
  • [8] Multidrug Resistant Gram-Negative Bacteria in Community-Acquired Pneumonia
    Cilloniz, Catia
    Dominedo, Cristina
    Torres, Antoni
    [J]. CRITICAL CARE, 2019, 23 (1)
  • [9] CLSI, 2015, PERFORMANCE STANDARD
  • [10] The Pel Polysaccharide Can Serve a Structural and Protective Role in the Biofilm Matrix of Pseudomonas aeruginosa
    Colvin, Kelly M.
    Gordon, Vernita D.
    Murakami, Keiji
    Borlee, Bradley R.
    Wozniak, Daniel J.
    Wong, Gerard C. L.
    Parsek, Matthew R.
    [J]. PLOS PATHOGENS, 2011, 7 (01)