Development of a Mass Sensitive Quartz Crystal Microbalance (QCM)-Based DNA Biosensor Using a 50 MHz Electronic Oscillator Circuit

被引：50

作者：

Garcia-Martinez, Gonzalo ^{[2
]}

Alonso Bustabad, Enrique ^{[2
]}

Perrot, Hubert ^{[1
,3
]}

Gabrielli, Claude ^{[1
,3
]}

Bucur, Bogdan ^{[1
,3
]}

Lazerges, Mathieu ^{[1
,3
]}

Rose, Daniel ^{[1
,3
]}

Rodriguez-Pardo, Loreto ^{[2
]}

Farina, Jose ^{[2
]}

Compere, Chantal ^{[4
]}

Arnau Vives, Antonio ^{[5
]}

机构：

[1] CNRS, LISE, UPR15, F-75252 Paris 05, France

[2] Univ Vigo, Dpto Tecnol Elect, Vigo 36310, Spain

[3] Univ Paris 06, F-75252 Paris 05, France

[4] IFREMER, Ctr Brest, Serv Interfaces & Capteurs, F-29280 Plouzane, France

[5] Univ Politecn Valencia, Wave Phenomena Grp, Dept Elect Engn, Valencia 46022, Spain

来源：

SENSORS | 2011年 / 11卷 / 08期

关键词：

DNA sequence detection; quartz crystal microbalance; electronic oscillator; sensitivity; frequency noise; resolution; SENSORS; RESOLUTION;

D O I：

10.3390/s110807656

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This work deals with the design of a high sensitivity DNA sequence detector using a 50 MHz quartz crystal microbalance (QCM) electronic oscillator circuit. The oscillator circuitry is based on Miller topology, which is able to work in damping media. Calibration and experimental study of frequency noise are carried out, finding that the designed sensor has a resolution of 7.1 ng/cm(2) in dynamic conditions (with circulation of liquid). Then the oscillator is proved as DNA biosensor. Results show that the system is able to detect the presence of complementary target DNAs in a solution with high selectivity and sensitivity. DNA target concentrations higher of 50 ng/mL can be detected.

引用

页码：7656 / 7664

页数：9

共 19 条

[1]

Allan D.W., 1966, P IEEE, V54, P211

[2]

[Anonymous], 1995, WID BANDW OP TRANSC

[3] DEVELOPMENT OF QUARTZ CRYSTAL-OSCILLATORS FOR UNDER-LIQUID SENSING [J].