type;
4;
fimbria;
fimbrial protein;
protein purification and sequencing;
Actinobacillus pleuropneumoniae;
D O I:
10.1016/S0378-1097(00)00244-5
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Type 4 fimbriae have been identified on the cell surface of Actinobacillus pleuropneumoniae by electron microscopy and N-terminal sequencing analysis. A. pleuropneumoniae type 4 fimbrial subunit protein, purified from cell cultures and from outer membrane preparations, reacted with polyclonal antibody raised against type 4 fimbriae of Moraxella bovis on Western blots. N-terminal sequence analysis of the purified 17 kDa type 4 fimbrial subunit protein, named ApfA, revealed the first 12 amino acids to be identical to those of other type 4 fimbrial subunit proteins. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.