Gene-expression Analysis of Acidic pH Shock Effects on Two-component Systems in Streptomyces coelicolor

被引：5

作者：

Kim, Yoon Jung ^{[1
]}

Moon, An Na ^{[1
]}

Song, Jae Yang ^{[1
]}

Kim, Eung Soo ^{[2
]}

Kim, Chang Joon ^{[3
]}

Chang, Yong Keun ^{[1
]}

机构：

[1] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South Korea

[2] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea

[3] Gyeongsang Natl Univ, Dept Chem Engn, Jinju 660701, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2009年 / 14卷 / 05期

关键词：

pH shock; Streptomyces coelicolor; transcriptional analysis; two-component system; SIGNAL-TRANSDUCTION SYSTEM; MORPHOLOGICAL-DIFFERENTIATION; SECONDARY METABOLISM; VANCOMYCIN; REGULATOR; ACTINORHODIN; CHITINASE; PRODUCT; OPERON; AFSR;

D O I：

10.1007/s12257-008-0260-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, transcriptional analyses by using reverse transcription-polymerase chain reaction (RT-PCR), DNA chip, and quantitative real time-PCR were performed to investigate effects of acidic pH shock on two-component systems in Streptomyces coelicolor A3(2). Two-component systems of cseC/cseB and vanS/vanR, known to be closely linked with self-protection against cell wall hydrolysis caused by external stimuli were upregulated by the pH shock. The chiS/chiR, afsQ2/afsQ1, ecrA2/ecrA1, bldM, ramC/ramR, and ragK/ragR known to be positively associated with the initiation of secondary metabolism were also upregulated. The cutS/cutR known to be negatively related to the secondary metabolism was, however, slightly downregulated. Upregulation or downregulation by the acidic pH shock of these two-component regulator systems might have contributed in a concerted manner to the enhancement of secondary metabolite production, at least, in this particular case of actinorhodin production in S. coelicolor A3(2). (C) KSBB

引用

页码：584 / 590

页数：7

共 29 条

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