Vaccinia virus N1L protein resembles a B cell lymphoma-2 (Bcl-2) family protein

被引：98
作者：
Aoyagi, Mika [1 ]
Zhai, Dayong [1 ]
Jin, Chaofang [1 ]
Aleshin, Alexander E. [1 ]
Stec, Boguslaw [1 ]
Reed, John C. [1 ]
Liddington, Robert C. [1 ]
机构：
[1] Brunham Inst Med Res, Infect & Inflammat Dis Ctr, La Jolla, CA 92037 USA
来源：
PROTEIN SCIENCE | 2007年 / 16卷 / 01期
关键词：
poxvirus; vaccinia virus; virulence; crystal structure; Bcl-2; apoptosis;
D O I：
10.1110/ps.062454707
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Poxviruses encode immuno-modulatory proteins capable of subverting host defenses. The poxvirus vaccinia expresses a small 14-kDa protein, N1L, that is critical for virulence. We report the crystal structure of N1L, which reveals an unexpected but striking resemblance to host apoptotic regulators of the B cell lymphoma-2 (Bcl-2) family. Although N1L lacks detectable Bcl-2 homology (BH) motifs at the sequence level, we show that N1L binds with high affinity to the BH3 peptides of pro-apoptotic Bcl-2 family proteins in vitro, consistent with a role for N1L in modulating host antiviral defenses.
引用
收藏
页码：118 / 124
页数：7
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