Vaccinia virus N1L protein resembles a B cell lymphoma-2 (Bcl-2) family protein

被引：99

作者：

Aoyagi, Mika ^{[1
]}

Zhai, Dayong ^{[1
]}

Jin, Chaofang ^{[1
]}

Aleshin, Alexander E. ^{[1
]}

Stec, Boguslaw ^{[1
]}

Reed, John C. ^{[1
]}

Liddington, Robert C. ^{[1
]}

机构：

[1] Brunham Inst Med Res, Infect & Inflammat Dis Ctr, La Jolla, CA 92037 USA

来源：

PROTEIN SCIENCE | 2007年 / 16卷 / 01期

关键词：

poxvirus; vaccinia virus; virulence; crystal structure; Bcl-2; apoptosis;

D O I：

10.1110/ps.062454707

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Poxviruses encode immuno-modulatory proteins capable of subverting host defenses. The poxvirus vaccinia expresses a small 14-kDa protein, N1L, that is critical for virulence. We report the crystal structure of N1L, which reveals an unexpected but striking resemblance to host apoptotic regulators of the B cell lymphoma-2 (Bcl-2) family. Although N1L lacks detectable Bcl-2 homology (BH) motifs at the sequence level, we show that N1L binds with high affinity to the BH3 peptides of pro-apoptotic Bcl-2 family proteins in vitro, consistent with a role for N1L in modulating host antiviral defenses.

引用

页码：118 / 124

页数：7

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