Expression of human epiregulin in E. coli

被引：0
|
作者：
Xi, QS [1 ]
Qian, XG [1 ]
Zhou, QW [1 ]
Pan, W [1 ]
Li, ZP [1 ]
Gan, RB [1 ]
机构：
[1] Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China
来源：
ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2000年 / 32卷 / 03期
关键词：
epiregulin; expression; purification; growth promotion; growth inhibition;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Human epiregulin cDNA was amplified from the lung cancer cell line A549 using RT-PCR. After adding 6 His codon to its 3' end, it was cloned into a high efficient secretive Escherichia coli system with alkaline phosphatase promoter( phoA promoter)constructed in our lab and induced for expression. The product was purified one-step by Ni-NTA column. Amino acid sequence analysis revealed the identity of our product with that previously reported. The product showed strong proliferative effect on fibroblast cell line Balb/c3T3 and growth inhibitory effect on epithelial carcinoma cell line A431.
引用
收藏
页码：295 / 298
页数：4
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