Cancer imaging using surface-enhanced resonance Raman scattering nanoparticles

被引:120
作者
Harmsen, Stefan [1 ]
Wall, Matthew A. [1 ,2 ,3 ]
Huang, Ruimin [1 ]
Kircher, Moritz F. [1 ,4 ,5 ,6 ]
机构
[1] Mem Sloan Kettering Canc Ctr, Dept Radiol, 1275 York Ave, New York, NY 10021 USA
[2] CUNY, Grad Ctr, Dept Chem, New York, NY USA
[3] CUNY Hunter Coll, New York, NY 10021 USA
[4] Mem Sloan Kettering Canc Ctr, Ctr Mol Imaging & Nanotechnol CMINT, 1275 York Ave, New York, NY 10021 USA
[5] Mem Sloan Kettering Canc Ctr, Dept Mol Pharmacol, 1275 York Ave, New York, NY 10021 USA
[6] Cornell Univ, Weill Cornell Med Coll, Dept Radiol, New York, NY 10021 USA
关键词
COATED GOLD NANOSTARS; SILICA NANOPARTICLES; IN-VIVO; SERS; SPECTROSCOPY; MODALITY; GROWTH; TAGS; CT;
D O I
10.1038/nprot.2017.031
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The unique spectral signatures and biologically inert compositions of surface-enhanced resonance Raman scattering (SERRS) nanoparticles make them promising contrast agents for in vivo cancer imaging. Our SERRS nanoparticles consist of a 60-nm gold nanoparticle core that is encapsulated in a 15-nm-thick silica shell wherein the resonant Raman reporter is embedded. Subtle aspects of their preparation can shift their limit of detection by orders of magnitude. In this protocol, we present the optimized, step-by-step procedure for generating reproducible SERRS nanoparticles with femtomolar (10(-15) M) limits of detection. We provide ways of characterizing the optical properties of SERRS nanoparticles using UV/VIS and Raman spectroscopy, and their physicochemical properties using transmission electron microscopy and nanoparticle tracking analysis. We introduce several applications of these nanoprobes for biomedical research, with a focus on intraoperative cancer imaging via Raman imaging. A detailed account is provided for successful i.v. administration of SERRS nanoparticles such that delineation of cancerous lesions can be achieved in vivo and ex vivo on resected tissues without the need for specific biomarker targeting. This straightforward, yet comprehensive, protocolfrom initial de novo gold nanoparticle synthesis to SERRS nanoparticle contrast-enhanced preclinical Raman imaging in animal modelstakes similar to 96 h.
引用
收藏
页码:1400 / 1414
页数:15
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