Mechanisms and effects of arsanilic acid on antibiotic resistance genes and microbial communities during pig manure digestion

被引：66

作者：

Sun, Wei ^{[1
]}

Qian, Xun ^{[1
]}

Gu, Jie ^{[1
,2
]}

Wang, Xiao-Juan ^{[1
]}

Zhang, Li ^{[1
]}

Guo, Ai-Yun ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Nat Resources & Environm, Yangling 712100, Shaanxi, Peoples R China

[2] Northwest A&F Univ, Res Ctr Recycle Agr Engn & Technol Shaanxi Prov, Yangling 712100, Shaanxi, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2017年 / 234卷

基金：

中国国家自然科学基金;

关键词：

Anaerobic digestion; Antibiotic resistance gene; Arsenic; Bacterial community; Human pathogenic bacteria; ANAEROBIC-DIGESTION; BACTERIAL PATHOGENS; PREVALENCE; METALS; IDENTIFICATION; TETRACYCLINE; SULFONAMIDE; MACROLIDE; ABUNDANCE; PLASMIDS;

D O I：

10.1016/j.biortech.2017.03.025

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

High concentrations of residual arsanilic acid occur in pig manure due to its use in feed to promote growth and control diseases. This study compared the effects of arsanilic acid at three concentrations (0, 325, and 650 mg/kg dry pig manure) on the abundance of antibiotic resistance genes (ARGs) and the microbial community during anaerobic digestion. Addition of 650 mg/kg arsanilic acid enhanced the absolute abundances of tetC, sul2, ermB, and gyrA more than twofold in the digestion product. Redundancy analysis indicated that the change in the microbial community structure was the main driver of variation in the ARGs profile. The As resistance gene arsC co-occurred with four ARGs and intI1, possibly causing the increase in ARGs under pressure by arsanilic acid. High arsanilic acid concentrations can increase the risk of ARGs occurring in anaerobic digestion products. The amount of arsanilic acid used as a feed additive should be controlled. (C) 2017 Published by Elsevier Ltd.

引用

页码：217 / 223

页数：7

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