De novo proteins from combinatorial libraries

被引：84
作者：
Moffet, DA [1 ]
Hecht, MH [1 ]
机构：
[1] Princeton Univ, Dept Chem, Princeton, NJ 08544 USA
来源：
CHEMICAL REVIEWS | 2001年 / 101卷 / 10期
关键词：
D O I：
10.1021/cr000051e
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The technique used for analyzing De Novo proteins from combinatorial libraries were discussed. The sequences targeted to form a tetrameric α-helical bundle was solved and compared with respect to the calculated structures. The results showed that the binary code strategy for protein design produced a large collection of de Novo sequences that folded into soluble α-helical structures.
引用
收藏
页码：3191 / 3203
页数：13
相关论文
共 102 条
[1]   RETRACTED: Directed evolution of new catalytic activity using the α/β-barrel scaffold (Retracted article. See vol 417, pg 468, 2002) [J].
Altamirano, MM ;
Blackburn, JM ;
Aguayo, C ;
Fersht, AR .
NATURE, 2000, 403 (6770) :617-622
[2]  
[Anonymous], 1992, ENZYME NOMENCLATURE, P862
[3]   SYNTHETIC POLYPEPTIDE WITH A COMPACT STRUCTURE AND ITS SELF-ORGANIZATION [J].
ANUFRIEVA, EV ;
BYCHKOVA, VE ;
KRAKOVYAK, MG ;
PAUTOV, VD ;
PTITSYN, OB .
FEBS LETTERS, 1975, 55 (01) :46-49
[4]   A heterodimeric coiled-coil peptide pair selected in vivo from a designed library-versus-library ensemble [J].
Arndt, KM ;
Pelletier, JN ;
Müller, KM ;
Alber, T ;
Michnick, SW ;
Plückthun, A .
JOURNAL OF MOLECULAR BIOLOGY, 2000, 295 (03) :627-639
[5]   Peptide helix induction in a self-assembling hemoprotein model [J].
Arnold, PA ;
Shelton, WR ;
Benson, DR .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (13) :3181-3182
[6]   Active barnase variants with completely random hydrophobic cores [J].
Axe, DD ;
Foster, NW ;
Fersht, AR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (11) :5590-5594
[7]  
Barbas 3rd C. F., 2001, PHAGE DISPLAY LAB MA
[8]   Protein design: The choice of de novo sequences [J].
Beasley, JR ;
Hecht, MH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (04) :2031-2034
[9]   Rational design of nascent metalloenzymes [J].
Benson, DE ;
Wisz, MS ;
Hellinga, HW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6292-6297
[10]   Construction of a novel redox protein by rational design: Conversion of a disulfide bridge into a mononuclear iron-sulfur center [J].
Benson, DE ;
Wisz, MS ;
Liu, WT ;
Hellinga, HW .
BIOCHEMISTRY, 1998, 37 (20) :7070-7076
12345678910