Knockdown of Rad51 expression induces radiation- and chemo-sensitivity in osteosarcoma cells

被引：27

作者：

Du, Li-Qing ^{[1
,2
]}

Wang, Yong ^{[1
,2
]}

Wang, Hong ^{[1
,2
]}

Cao, Jia ^{[1
,2
]}

Liu, Qiang ^{[1
,2
]}

Fan, Fei-Yue ^{[1
,2
]}

机构：

[1] Chinese Acad Med Sci, Inst Radiat Med, Tianjin 300192, Peoples R China

[2] Peking Union Med Coll, Tianjin Key Lab Mol Nucl Med, Tianjin 300192, Peoples R China

来源：

MEDICAL ONCOLOGY | 2011年 / 28卷 / 04期

关键词：

Rad51; Osteosarcoma; RNA interference; Radiosensitivity; Chemosensitivity; LUNG-CANCER CELLS; HOMOLOGOUS RECOMBINATION; IONIZING-RADIATION; PROTEIN; OVEREXPRESSION; CYTOTOXICITY; SUPPRESSION; INHIBITION; RESISTANCE; GEFITINIB;

D O I：

10.1007/s12032-010-9605-1

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Osteosarcoma is the common primary bone malignancy in children and young adults in Eastern countries. Resistance to ionizing radiation (IR) or drugs is an underlying mechanism contributing to the failure of therapy in these patients. Rad51 is the key protein of DNA homologous recombination repair. Although high expression of Rad51 is associated with enhanced resistance to DNA damage induced by chemicals and/or ionizing radiation, the relevance of Rad51 expression in osteosarcoma and its relationship with IR sensitivity and chemo-resistance is not well understood. In this study, we elucidated the possibility of using Rad51 in the treatment of human osteosarcoma in vitro. Changes in chemo- and radiation sensitivity in cultured osteosarcoma cells occurred after suppression of Rad51 expression, using a plasmid vector-mediated short hairpin RNA (shRNA) expression system. The suppression of Rad51 correlated with cell cycle arrest in the G(2) phase and inhibited tumor cell proliferation. Our results suggest that Rad51 expression levels might play an important role in radiation- and chemo-sensitivity of human osteosarcoma.

引用

页码：1481 / 1487

页数：7