Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species

被引：179

作者：

Bossi, Mariano ^{[1
]}

Foelling, Jonas ^{[1
]}

Belov, Vladimir N. ^{[1
]}

Boyarskiy, Vadim P. ^{[1
]}

Medda, Rebecca ^{[1
]}

Egner, Alexander ^{[1
]}

Eggeling, Christian ^{[1
]}

Schoenle, Andreas ^{[1
]}

Hell, Stefan W. ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept NanoBiophoton, D-37077 Gottingen, Germany

来源：

NANO LETTERS | 2008年 / 8卷 / 08期

关键词：

D O I：

10.1021/nl801471d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques.

引用

页码：2463 / 2468

页数：6

共 43 条

[1]

Abbe E., 1873, ARCH MIKROSK ANAT, V9, P413, DOI [10.1007/BF02956173, DOI 10.1007/BF02956173]

[2] Multicolor super-resolution imaging with photo-switchable fluorescent probes [J].

Bates, Mark ;

Huang, Bo ;

Dempsey, Graham T. ;

Zhuang, Xiaowei .

SCIENCE, 2007, 317 (5845) :1749-1753

[3] PROPOSED METHOD FOR MOLECULAR OPTICAL IMAGING [J].

BETZIG, E .

OPTICS LETTERS, 1995, 20 (03) :237-239

[4] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[5] POSITION MEASUREMENT WITH A RESOLUTION AND NOISE-LIMITED INSTRUMENT [J].

BOBROFF, N .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1986, 57 (06) :1152-1157

[6] Two-color far-field fluorescence nanoscopy based on photoswitchable emitters [J].

Bock, H. ;

Geisler, C. ;

Wurm, C. A. ;

Von Middendorff, C. ;

Jakobs, S. ;

Schoenle, A. ;

Egner, A. ;

Hell, S. W. ;

Eggeling, C. .

APPLIED PHYSICS B-LASERS AND OPTICS, 2007, 88 (02) :161-165

[7] Photostable, amino reactive and water-soluble fluorescent labels based on sulfonated rhodamine with a rigidized xanthene fragment [J].

Boyarskiy, Vadim P. ;

Belov, Vladimir N. ;

Medda, Rebecca ;

Hein, Birka ;

Bossi, Mariano ;

Hell, Stefan W. .

CHEMISTRY-A EUROPEAN JOURNAL, 2008, 14 (06) :1784-1792

[8] Two-color far-field fluorescence nanoscopy [J].

Donnert, Gerald ;

Keller, Jan ;

Wurm, Christian A. ;

Rizzoli, Silvio O. ;

Westphal, Volker ;

Schoenle, Andreas ;

Jahn, Reinhard ;

Jakobs, Stefan ;

Eggeling, Christian ;

Hell, Stefan W. .

BIOPHYSICAL JOURNAL, 2007, 92 (08) :L67-L69

[9] Macromolecular-scale resolution in biological fluorescence microscopy [J].

Donnert, Gerald ;

Keller, Jan ;

Medda, Rebecca ;

Andrei, M. Alexandra ;

Rizzoli, Silvio O. ;

Luehrmann, Reinhard ;

Jahn, Reinhard ;

Eggeling, Christian ;

Hell, Stefan W. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (31) :11440-11445

[10] Immunofluorescence stimulated emission depletion microscopy [J].

Dyba, M ;

Jakobs, S ;

Hell, SW .

NATURE BIOTECHNOLOGY, 2003, 21 (11) :1303-1304

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