Cyclosporin A inhibits the replication of diverse coronaviruses

被引：183

作者：

de Wilde, Adriaan H. ^{[1
]}

Zevenhoven-Dobbe, Jessika C. ^{[1
]}

van der Meer, Yvonne ^{[1
]}

Thiel, Volker ^{[2
,3
]}

Narayanan, Krishna ^{[4
]}

Makino, Shinji ^{[4
]}

Snijder, Eric J. ^{[1
]}

van Hemert, Martijn J. ^{[1
]}

机构：

[1] Leiden Univ, Med Ctr, Ctr Infect Dis, Mol Virol Lab,Dept Med Microbiol, Leiden, Netherlands

[2] Kantonal Hosp St Gallen, Inst Immunobiol, St Gallen, Switzerland

[3] Univ Zurich, Vetsuisse Fac, Zurich, Switzerland

[4] Univ Texas Med Branch, Dept Microbiol & Immunol, Galveston, TX USA

来源：

JOURNAL OF GENERAL VIROLOGY | 2011年 / 92卷

基金：

瑞士国家科学基金会;

关键词：

C VIRUS-REPLICATION; RESPIRATORY SYNDROME CORONAVIRUS; HEPATITIS-C; POTENT INHIBITOR; CYCLOPHILIN; CHLOROQUINE; DEBIO-025; INFECTION; PROTEIN;

D O I：

10.1099/vir.0.034983-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Low micromolar, non-cytotoxic concentrations of cyclosporin A (CsA) strongly affected the replication of severe acute respiratory syndrome coronavirus (SARS-CoV), human coronavirus 229E and mouse hepatitis virus in cell culture, as was evident from the strong inhibition of GFP reporter gene expression and a reduction of up to 4 logs in progeny titres. Upon high-multiplicity infection, CsA treatment rendered SARS-CoV RNA and protein synthesis almost undetectable, suggesting an early block in replication. siRNA-mediated knockdown of the expression of the prominent CsA targets cyclophilin A and B did not affect SARS-CoV replication, suggesting either that these specific cyclophilin family members are dispensable or that the reduced expression levels suffice to support replication.

引用

页码：2542 / 2548

页数：7

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