DETERMINATION OF FENBENDAZOLE IN ADULT ARTEMIA USING REVERSE-PHASE LIQUID CHROMATOGRAPHY

被引：0
作者：
Cox, Sherry [1 ]
Allender, Matthew C. [2 ]
Yarbrough, Jason [1 ]
机构：
[1] Univ Tennessee, Dept Comparat Med, Coll Vet Med, Knoxville, TN 37996 USA
[2] Univ Tennessee, Dept Small Anim Clin Sci, Coll Vet Med, Knoxville, TN 37996 USA
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2011年 / 34卷 / 15期
关键词：
anthelmintics; aquaculture; artemia; bioencapsulation; fenbendazole; HPLC; BONE-MARROW HYPOPLASIA; PLASMA DISPOSITION; BRINE SHRIMP; METABOLITES; BIOENCAPSULATION; OXFENDAZOLE; ALBENDAZOLE; CATTLE;
D O I：
10.1080/10826076.2011.575977
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new HPLC procedure for the determination of fenbendazole, a benzimidazole, in adult Artemia (live brine shrimp) has been developed and validated. Following a liquid extraction using ethyl acetate, samples were separated by reversed-phase HPLC on a Sunfire C-18 column and quantified using UV detection at 215 nm. The mobile phase was a mixture of 37.5 mM potassium dihydrogen phosphate pH 3.75 and acetonitrile, with a flow-rate of 0.9 mL/min. The procedure produced a linear curve over the concentration range of 100-5000 mu g/gm with a lower limit of quantification of 1 mu g/gm. Intra- and inter-assay variability was less than 8%. The development of the assay allowed the determination of fenbendazole in a live brine shrimp feeding study.
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收藏
页码：1536 / 1545
页数：10
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