Synthesis of lab-in-a-pipette-tip extraction using hydrophilic nano-sized dummy molecularly imprinted polymer for purification and analysis of prednisolone

被引:68
作者
Arabi, Maryam [1 ]
Ghaedi, Mehrorang [1 ]
Ostovan, Abbas [2 ]
Wang, Shaobin [3 ]
机构
[1] Univ Yasuj, Dept Chem, Yasuj 7591435, Iran
[2] Islamic Azad Univ, Kerman Branch, Dept Chem, Kerman, Iran
[3] Curtin Univ, Dept Chem Engn, GPO Box U1987, Perth, WA 6845, Australia
关键词
Molecularly imprinted polymer; Dummy template; Pipette-tip solid phase extraction; Prednisolone; SOLID-PHASE EXTRACTION; MICELLAR ELECTROKINETIC CHROMATOGRAPHY; PERFORMANCE LIQUID-CHROMATOGRAPHY; COVALENT IMMOBILIZATION; FUNCTIONALIZED SILICA; HUMAN-PLASMA; NANOPARTICLES; MILK; PHARMACOKINETICS; AMOXICILLIN;
D O I
10.1016/j.jcis.2016.07.017
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
A novel pipette-tip based on nano-sized dummy molecularly imprinted polymer (PT-DMIP) assisted by ultrasonication for the effective enrichment and analysis of prednisolone from urine samples was developed. The PT-DMIP cartridge was prepared by packing the dummy molecularly imprinted polymer at the tip of the micropipette. The polymerization used betamethasone (BM) as the dummy template, 3-aminopropyltrimethoxysilane (APTMS) as the functionalized monomer, tetraethyl orthosilicate (TEOS) as the cross-linker and aluminum ion (Al3+) as a dopant to produce Lewis acid sites in the silica matrix for metal coordinative interactions with the analyte. Compared to conventional solid phase extraction (SPE), the PT-DMIP is cost-effective, fast, and easy to handle, while the system is very approachable and reduces the consumption of toxic organic solvent. HPLC-UV analysis revealed successful applicability of the sorbent for highly efficient extraction of perdnisolone from urine matrices. The extraction recovery was investigated and optimum conditions were obtained using central composite design. Good linearity for prednisolone in the range of 0.22-220 mu g L-1 with regression coefficients of 0.99 reveals high applicability of the method for trace analysis. Under the optimized conditions, the recoveries are 89.0-96.1 with relative standard deviations (RSD) of less than 9.0%. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码:232 / 239
页数:8
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