Mesenchymal stem cells from human bone marrow and adipose tissue: Isolation, characterization, and differentiation potentialities

被引：138
|
作者：
Romanov, YA [1 ]
Darevskava, AN [1 ]
Merzlikina, NV [1 ]
Buravkova, LB [1 ]
机构：
[1] Russian Acad Sci, Inst Biomed Problems, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2005年 / 140卷 / 01期
关键词：
mesenchymal stem cells; cell culture; bone marrow; lipoaspirate; differentiation; phenotype;
D O I：
10.1007/s10517-005-0430-z
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Comparative study of cultured human bone marrow and adipose tissue (lipoaspirate) mesenchymal stem cells was carried out. The main morphological parameters, proliferative activity, expression of surface and intracellular markers of these cells were characterized. Flow cyto-fluorometry and histological staining showed that both cell types exhibited similar expression of CD105, CD54, CD106, HLA-I markers, were positively stained for vimentin, ASMA, collagen-1, and fibronectin, but not HLA-DR, CD 117, and hemopoietic cell markers. The cells underwent differentiation into adipocytes and osteoblasts under appropriate conditions of culturing. Incubation under neuroinductive conditions led to the appearance of a cell population positively stained for type III beta-tubulin (neuronal differentiation marker).
引用
收藏
页码：138 / 143
页数：6
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