An alternative workflow for molecular detection of SARS-CoV-2-escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020

被引：136

作者：

Fomsgaard, Anna S. ^{[1
]}

Rosenstierne, Maiken Worsoe ^{[1
]}

机构：

[1] Statens Serum Inst, Dept Virus & Microbiol Special Diagnost, Copenhagen, Denmark

来源：

EUROSURVEILLANCE | 2020年 / 25卷 / 14期

基金：

欧盟地平线“2020”;

关键词：

D O I：

10.2807/1560-7917.ES.2020.25.14.2000398

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 degrees C before a commonly-used RT-qPCR procedure.

引用

页码：6 / 9

页数：4

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