Integrated high capacity solid phase extraction-MS/MS system for pharmaceutical profiling in drug discovery

被引：21
作者：
Kerns, EH
Kleintop, T
Little, D
Tobien, T
Mallis, L
Di, L
Hu, M
Hong, Y
McConnell, OJ
机构：
[1] Wyeth Ayerst Res, Princeton, NJ 08543 USA
[2] Micromass, Manchester, Lancs, England
[3] LEAO Technol, Carrboro, NC USA
[4] Wyeth Ayerst Res, Collegeville, PA USA
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2004年 / 34卷 / 01期
关键词：
drug discovery; stability; high throughput; MS/MS; on-line solid phase extraction; screening; pharmaceutical profiling;
D O I：
10.1016/j.japna.2003.03.001
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method is described for use in analysis of samples from pharmaceutical profiling of early drug discovery compounds. The method consists of a high capacity autosampler which injects samples into one of two solid phase extraction columns operated in parallel for alternating trapping, washing and elution into a tandem quadrupole mass spectrometer operated in multiple reaction monitoring (MRM) MS/MS mode. A primary method, which is useful for 80-90% of compounds, and a secondary method, which is useful for a majority of the remaining compounds, are described. No analytical HPLC column is used and the analysis rate is approximately 50 samples/h. Specificity is obtained using MRM analysis. Application of the method for high capacity analysis of metabolic stability samples is described. (C) 2003 Elsevier B.V. All rights reserved.
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页码：1 / 9
页数：9
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