Antioxidant and Antimicrobial Activities of Chemically-Characterized Essential Oil from Artemisia aragonensis Lam. against Drug-Resistant Microbes

被引：62

作者：

Chebbac, Khalid ^{[1
]}

Ghneim, Hazem K. ^{[2
]}

El Moussaoui, Abdelfattah ^{[3
]}

Bourhia, Mohammed ^{[4
]}

El Barnossi, Azeddin ^{[3
]}

Ouaritini, Zineb Benziane ^{[5
]}

Salamatullah, Ahmad Mohammad ^{[6
]}

Alzahrani, Abdulhakeem ^{[6
]}

Aboul-Soud, Mourad A. M. ^{[2
]}

Giesy, John P. ^{[7
,8
,9
,10
]}

Guemmouh, Raja ^{[1
]}

机构：

[1] Sidi Mohammed Ben Abdallah Univ, Fac Sci Dhar El Mahraz, Lab Biotechnol Conservat & Valorisat Nat Resource, Fes 30000, Morocco

[2] King Saud Univ, Dept Clin Lab Sci, Coll Appl Med Sci, POB 10219, Riyadh 11433, Saudi Arabia

[3] Sidi Mohammed Ben Abdellah Univ, Fac Sci Dhar El Mahraz, Lab Biotechnol Environm Agrifood & Hlth, Fes 30000, Morocco

[4] Univ Hassan 2, Fac Med & Pharm, Lab Chem Biochem Nutr & Environm, Casablanca 20000, Morocco

[5] Sidi Mohamed Ben Abdellah Univ, Lab Nat Subst Pharmacol Environm Modeling Hlth &, Fac Sci, Fes 30000, Morocco

[6] King Saud Univ, Dept Food Sci & Nutr, Coll Food & Agr Sci, POB 2460, Riyadh 11451, Saudi Arabia

[7] Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada

[8] Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B3, Canada

[9] Michigan State Univ, Dept Integrat Biol, Ctr Integrat Toxicol, E Lansing, MI 48824 USA

[10] Baylor Univ, Dept Environm Sci, Waco, TX 76706 USA

来源：

MOLECULES | 2022年 / 27卷 / 03期

关键词：

essential oils; phytochemical analysis; antioxidant; antibacterial; antimicrobial resistance; PLANT ESSENTIAL OILS; ANTIBACTERIAL ACTIVITY; CANDIDAL INFECTIONS; PHENOLIC-COMPOUNDS; L;

D O I：

10.3390/molecules27031136

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study investigated the chemical composition, antioxidant and antimicrobial activity of essential oil extracted from Artemisia aragonensis Lam. (EOA). Hydrodistillation was employed to extract EOA. Gas chromatography with flame ionization detection (GC-FID) and gas chromatography-mass spectrometry analyses (GC-MS) were used to determine the phytochemical composition of EOA. Antioxidant potential was examined in vitro by use of three tests: 2.2-diphenyl-1-picrilhidrazil (DPPH), ferric reducing activity power (FRAP) and total antioxidant capacity assay (TAC). Agar diffusion and microdilution bioassays were used to assess antimicrobial activity. GC/MS and GC-FID detected 34 constituents in the studied EOA. The major component was Camphor (24.97%) followed by Borneol (13.20%), 1,8 Cineol (10.88%), and Artemisia alcohol (10.20%). EOA exhibited significant antioxidant activity as measured by DPPH and FRAP assays, with IC50 and EC50 values of 0.034 +/- 0.004 and 0.118 +/- 0.008 mg/mL, respectively. EOA exhibited total antioxidant capacity of 7.299 +/- 1.774 mg EAA/g. EOA exhibited potent antibacterial activity as judged by the low minimum inhibitory concentration (MIC) values against selected clinically-important pathogenic bacteria. MIC values of 6.568 +/- 1.033, 5.971 +/- 1.033, 7.164 +/- 0.0 and 5.375 +/- 0.0 mu g/mL were observed against S. aureus, B. subtills, E. coli 97 and E. coli 57, respectively. EOA displayed significant antifungal activity against four strains of fungi: F. oxysporum, C. albicans, A. flavus and A. niger with values of 21.50 +/- 0.43, 5.31 +/- 0.10, 21.50 +/- 0.46 and 5.30 +/- 0.036 mu g/mL, respectively. The results of the current study highlight the importance of EOA as an alternative source of natural antioxidant and antibacterial drugs to combat antibiotic-resistant microbes and free radicals implicated in the inflammatory responses accompanying microbial infection.

引用

页数：15

共 66 条

[1] Insecticidal and Antifungal Activities of Chemically-Characterized Essential Oils from the Leaves of Withania frutescens L. [J].