Enzyme electrodes for determination of total phenolic capacity of red wines

被引：4
|
作者：
Kiralp, S [1 ]
Cirpan, A [1 ]
Toppare, L [1 ]
机构：
[1] Middle E Tech Univ, Dept Chem, TR-06531 Ankara, Turkey
来源：
JOURNAL OF APPLIED POLYMER SCIENCE | 2005年 / 98卷 / 01期
关键词：
electrochemistry; enzymes; copolymerization; conducting polymers;
D O I：
10.1002/app.22165
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
A tyrosinase-modified electrode, based on physical entrapment of enzyme in copolymer matrix of decanedioic acid bis(4-pyrrol-1-yl-phenyl) ester (DAPE) with pyrrole was constructed and used to investigate the total amount of phenolics in red wines. Kinetic parameters, V-max, and K-m, were determined. Also, temperature, pH optimizations, operational stability and shelf life of the enzyme electrode were examined. Surface morphologies of the films were studied by scanning electron microscope. (c) 2005 Wiley Periodicals, Inc.
引用
收藏
页码：521 / 524
页数：4
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