Expression and characterization of vanilloid receptor subtype 1 in human dental pulp cell cultures

被引：39

作者：

Miyamoto, R ^{[1
]}

Tokuda, M ^{[1
]}

Sakuta, T ^{[1
]}

Nagaoka, S ^{[1
]}

Torii, M ^{[1
]}

机构：

[1] Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Restorat Dent & Endodontol, Kagoshima 8908544, Japan

来源：

JOURNAL OF ENDODONTICS | 2005年 / 31卷 / 09期

关键词：

VR1; capsaicin; IL-6; mitogen-activated protein kinases; NF-kappa B;

D O I：

10.1097/01.don.0000155259.22746.ae

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The expression of the vanilloid receptor subtype 1 (VR1, TRPV1) was detected in human dental pulp fibroblasts (PF-10) using RT-PCR, Western blotting, and immunocytochemical analysis. As revealed by ELISA, capsaicin induced IL-6 expression in PF-10 cells, and the VR1 antagonist capsazepine dose-dependently inhibited capsaicin-incluced IL-6 production, indicating that capsaicin-induced IL-6 expression is related to VR1 activation. The interaction between capsaicin and mitogenactivated protein kinases (MAPKs) was investigated. The phosphorylation of p38 MAPK and c-Jun NH2-terminal kinase (JNK) were detected after capsaicin stimulation. p38 MAPK is involved in capsaicin-induced IL-6 production, as shown by the use of specific inhibitors of this kinase. The result of EMSA showed that capsaicin inhibited tumor necrosis factor-alpha (TNF alpha)-induced nuclear factor-kappa B (NF-kappa B) activation in PF-10 cell cultures. These results suggest that the activation of VR1 plays an important role in dental pulp inflammation.

引用

页码：652 / 658

页数：7

共 33 条

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