Efficient skin permeation of soluble proteins via flexible and functional nano-carrier

被引:82
作者
Choi, Won Il [1 ,2 ]
Lee, Jong Hyun [1 ,2 ]
Kim, Ja-Young [1 ,2 ]
Kim, Jin-Chul [3 ,4 ]
Kim, Young Ha [1 ,2 ]
Tae, Giyoong [1 ,2 ]
机构
[1] Gwangju Inst Sci & Technol, Sch Mat Sci & Engn, Kwangju 500712, South Korea
[2] Gwangju Inst Sci & Technol, Dept Nanobio Mat & Elect, Kwangju 500712, South Korea
[3] Kangwon Natl Univ, Sch Biotechnol & Bioengn, Chunchon 200701, Kangwon Do, South Korea
[4] Kangwon Natl Univ, Inst Biosci & Biotechnol, Chunchon 200701, Kangwon Do, South Korea
关键词
Pluronic; Nano-carrier; Chitosan; Proteins; Transdermal; N-TRIMETHYL CHITOSAN; TRANSDERMAL DELIVERY; BARRIER FUNCTION; IN-VITRO; PENETRATION; INSULIN; ABSORPTION; ULTRASOUND; IONTOPHORESIS; PERMEABILITY;
D O I
10.1016/j.jconrel.2011.08.013
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In spite of several intrinsic and distinct advantages, a topical and transdermal administration of drugs has been limited mainly due to very low permeability of drugs across skin. Especially, it is generally regarded that hydrophilic macromolecules such as proteins, peptides, and vaccines cannot penetrate across skin. In this study, we demonstrated that chitosan-conjugated, Pluronic-based nano-carrier (nanogel) can act as an efficient delivery vehicle of hydrophilic proteins across human skin. The functional nano-carrier (<100 nm in size), chemically-crosslinking Pluronic F 127 with chitosan conjugation, is flexible and soft with reservoir characteristics for biomacromolecules. The in-vitro permeation experiments through human cadaver skin revealed remarkable permeability of hydrophilic proteins of various sizes including FITC-BSA (67 kDa) and FITC-Insulin (6 kDa) by direct penetration of the nano-carrier across skin. The bioactivity post-permeation of proteins via the functional nano-carrier was also confirmed by delivering beta-galactosidase. Results presented in this paper suggest the use of chitosan-conjugated flexible nano-carrier as a novel platform for transcutaneous delivery of hydrophilic macromolecules and other drug-delivery applications. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:272 / 278
页数:7
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