CREB1-induced lncRNA LEF1-AS1 contributes to colorectal cancer progression via the miR-489/DIAPH1 axis

被引：19
作者：
Cheng, Yan [1 ]
Wu, Jing [1 ]
Qin, Bin [1 ]
Zou, Bai-cang [1 ]
Wang, Yong-hua [1 ]
Li, Yang [2 ]
机构：
[1] Xi An Jiao Tong Univ, Dept Digest Dis, Affiliated Hosp 2, Xian, Shaanxi, Peoples R China
[2] Xi An Jiao Tong Univ, Dept Otolaryngol Head & Neck Surg, Affiliated Hosp 2, 157 Xiwu Rd, Xian 710004, Shaanxi, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2020年 / 526卷 / 03期
关键词：
LncRNA LEF1-AS1; miR-489; DIAPH1; Biomarker; Colorectal cancer; Metastasis; LONG NONCODING RNAS; STATISTICS;
D O I：
10.1016/j.bbrc.2020.03.153
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Long non-coding RNAs (lncRNAs) have been identified as new regulatory factors in tumor progression. Lymphoid enhancer-binding factor 1 antisense RNA 1 (LEF1-AS1) was a recently identified lncRNA. This research aimed to investigate the roles and mechanisms of LEF1-AS1 in colorectal cancer (CRC). We firstly showed that LEF1-AS1 expression was upregulated in human CRC tissues and cell lines. LEF1-AS1 upregulation was demonstrated to be induced by CREB1. Clinical study revealed that high LEF1-AS1 expression was positively associated with histological grade, lymph nodes metastasis, and decreased survivals of CRC patients. Functionally, down-regulation of LEF1-AS1 using si-LEF1-AS1 decreased cell growth, migration and invasion, as well as increased apoptosis in CRC cells. Mechanically, LEF1-AS1 functioned as competing endogenous RNA (ceRNA) for miR-489 to positively recover DIAPH1, thus playing an oncogenic role in CRC pathogenesis. Overall, our observations identified a novel CRC-related lncRNA LEF1-AS1 and discovered a critical role for this lncRNA as a ceRNA in CRC pathogenesis, suggesting that it may serve as a novel biomarker for prognosis and act as a therapeutic target for CRC treatment. (C) 2020 Elsevier Inc. All rights reserved.
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收藏
页码：678 / 684
页数：7
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