High-throughput scanning confocal microscope for single molecule analysis

被引：23

作者：

Sabanayagam, CR ^{[1
]}

Eid, JS ^{[1
]}

Meller, A ^{[1
]}

机构：

[1] Harvard Univ, Rowland Inst, Cambridge, MA 02142 USA

来源：

APPLIED PHYSICS LETTERS | 2004年 / 84卷 / 07期

关键词：

D O I：

10.1063/1.1646725

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

An automated and programmable confocal microscope that can acquire upwards of 10(3) single-molecule fluorescence resonance energy transfer (FRET) time traces is presented. The microscope augments the capabilities of current instruments by employing a flow cell that allows full control of the sample's chemical environment, automatic locating and probing of single molecules, and an automatic focusing feature that enables the unattended scanning of large areas of the sample. This microscope performs routine acquisition of thousands of single-molecule events, in a highly reproducible fashion. These capabilities were utilized in recording the distributions of FRET efficiencies arising from control DNA molecules specifically labeled with internal donor-acceptor reporters. (C) 2004 American Institute of Physics.

引用

页码：1216 / 1218

页数：3

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