Human iPS cell derived RPE strips for secure delivery of graft cells at a target place with minimal surgical invasion

被引:27
作者
Nishida, Mitsuhiro [1 ]
Tanaka, Yuji [1 ,2 ]
Tanaka, Yo [3 ]
Amaya, Satoshi [3 ]
Tanaka, Nobuyuki [3 ]
Uyama, Hirofumi [1 ,4 ]
Masuda, Tomohiro [1 ,6 ]
Onishi, Akishi [1 ]
Sho, Junki [1 ,5 ]
Yokota, Satoshi [1 ,4 ]
Takahashi, Masayo [1 ,4 ,5 ,6 ]
Mandai, Michiko [1 ,4 ]
机构
[1] RIKEN Ctr Biosyst Dynam Res, Lab Retinal Regenerat, Chuo Ku, 2-2-3 Minatojima Minamimachi, Kobe, Hyogo 6500047, Japan
[2] Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Div Med, Kofu, Yamanashi, Japan
[3] RIKEN Ctr Biosyst Dynam Res, Lab Integrated Biodevice, Kobe, Hyogo, Japan
[4] Kobe City Eye Hosp, Dept Ophthalmol, Kobe, Hyogo, Japan
[5] Vis Care Inc, Kobe, Hyogo, Japan
[6] Vis Care Cell Therapy Inc, Kobe, Hyogo, Japan
关键词
PLURIPOTENT STEM-CELLS; MACULAR DEGENERATION; DRIVEN;
D O I
10.1038/s41598-021-00703-x
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Several clinical studies have been conducted into the practicality and safety of regenerative therapy using hESC/iPSC-retinal pigment epithelium (RPE) as a treatment for the diseases including age-related macular degeneration. These studies used either suspensions of RPE cells or an RPE cell sheet. The cells can be injected using a minimally invasive procedure but the delivery of an intended number of cells at an exact target location is difficult; cell sheets take a longer time to prepare, and the surgical procedure is invasive but can be placed at the target area. In the research reported here, we combined the advantages of the two approaches by producing a quickly formed hiPSC-RPE strip in as short as 2 days. The strip readily expanded into a monolayer sheet on the plate, and after transplantation in nude rats, it showed a potency to partly expand with the correct apical/basal polarity in vivo, although limited in expansion area in the presence of healthy host RPE. The strip could be injected into a target area in animal eyes using a 24G canula tip.
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页数:14
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