Sequencing of adenine in DNA by scanning tunneling microscopy

被引：7

作者：

Tanaka, Hiroyuki ^{[1
]}

Taniguchi, Masateru ^{[1
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, Osaka 5670047, Japan

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS | 2017年 / 56卷 / 08期

关键词：

DEOXYRIBONUCLEIC-ACID; CU(111) SURFACES; BASE MOLECULES; ELECTRONIC-PROPERTIES; SPECTROSCOPY; OLIGOMER;

D O I：

10.7567/JJAP.56.08LB02

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

The development of DNA sequencing technology utilizing the detection of a tunnel current is important for next-generation sequencer technologies based on single-molecule analysis technology. Using a scanning tunneling microscope, we previously reported that dI/dV measurements and dI/dV mapping revealed that the guanine base (purine base) of DNA adsorbed onto the Cu(111) surface has a characteristic peak at Vs = % 1.6 V. If, in addition to guanine, the other purine base of DNA, namely, adenine, can be distinguished, then by reading all the purine bases of each single strand of a DNA double helix, the entire base sequence of the original double helix can be determined due to the complementarity of the DNA base pair. Therefore, the ability to read adenine is important from the viewpoint of sequencing. Here, we report on the identification of adenine by STM topographic and spectroscopic measurements using a synthetic DNA oligomer and viral DNA. (C) 2017 The Japan Society of Applied Physics

引用

页数：3

共 29 条

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