An N-terminal motif in NLR immune receptors is functionally conserved across distantly related plant species

被引：157

作者：

Adachi, Hiroaki ^{[1
]}

Contreras, Mauricio P. ^{[1
]}

Harant, Adeline ^{[1
]}

Wu, Chih-hang ^{[1
]}

Derevnina, Lida ^{[1
]}

Sakai, Toshiyuki ^{[1
]}

Duggan, Cian ^{[2
]}

Moratto, Eleonora ^{[2
]}

Bozkurt, Tolga O. ^{[2
]}

Maqbool, Abbas ^{[1
]}

Win, Joe ^{[1
]}

Kamoun, Sophien ^{[1
]}

机构：

[1] Univ East Anglia, Sainsbury Lab, Norwich Res Pk, Norwich, Norfolk, England

[2] Imperial Coll London, Dept Life Sci, London, England

来源：

ELIFE | 2019年 / 8卷

基金：

欧洲研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

PHYTOPHTHORA-INFESTANS; COILED-COIL; RESISTANCE; REVEALS; POTATO; ACTIVATION; EFFECTOR; DEFENSE; DOMAINS; DEATH;

D O I：

10.7554/eLife.49956

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The molecular codes underpinning the functions of plant NLR immune receptors are poorly understood. We used in vitro Mu transposition to generate a random truncation library and identify the minimal functional region of NLRs. We applied this method to NRC4-a helper NLR that functions with multiple sensor NLRs within a Solanaceae receptor network. This revealed that the NRC4 N-terminal 29 amino acids are sufficient to induce hypersensitive cell death. This region is defined by the consensus MADAxVSFxVxKLxxLLxxEx (MADA motif) that is conserved at the N-termini of NRC family proteins and similar to 20% of coiled-coil (CC)-type plant NLRs. The MADA motif matches the N-terminal alpha 1 helix of Arabidopsis NLR protein ZAR1, which undergoes a conformational switch during resistosome activation. Immunoassays revealed that the MADA motif is functionally conserved across NLRs from distantly related plant species. NRC-dependent sensor NLRs lack MADA sequences indicating that this motif has degenerated in sensor NLRs over evolutionary time.

引用

页数：31

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