Use of multilocus methylation-specific single nucleotide primer extension (MS-SNuPE) technology in diagnostic testing for human imprinted loci

被引:25
作者
Begemann, Matthias [1 ]
Leisten, Isabelle [1 ]
Soellner, Lukas [1 ]
Zerres, Klaus [1 ]
Eggermann, Thomas [1 ]
Spengler, Sabrina [1 ]
机构
[1] Rhein Westfal TH Aachen, Inst Human Genet, D-52062 Aachen, Germany
关键词
MS-SNuPE; methylation testing; multilocus methylation defects; Silver-Russell syndrome; Beckwith-Wiedemann syndrome; EPIGENETIC ALTERATIONS; HYPOMETHYLATION; SILVER; REVEALS;
D O I
10.4161/epi.19719
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A number of diseases have been found to be linked to aberrant methylation of specific genes. However, most of the routine diagnostic techniques to detect epigenetic disturbances are restricted to single loci. Additionally, a precise quantification of the methylation status is often hampered. A considerable fraction of patients with Silver-Russell syndrome, Beckwith-Wiedemann syndrome and transient neonatal diabetes mellitus exhibit loss of methylation at further imprinted loci in addition to the disease specific ones (multilocus methylation defects, MLMD). As the currently available tests are mainly focused on single imprinted loci on different chromosomes and thereby make the detection of multilocus methylation defects time-consuming and expensive, we established methylation-specific single nucleotide primer extension (MS-SNuPE) assays for a simultaneous quantification of methylation at multiple methylated loci. We chose loci generally affected in patients with MLMD. The method was validated by screening 66 individuals with known (epi)genetic disturbances. In comparison to other methylation-specific techniques, multilocus methylation-specific single nucleotide primer extension allows the quantitative analysis of numerous CpG islands of different loci in one assay and is, therefore, suitable for the simultaneous diagnostic testing for different congenital imprinting disorders in parallel, as well as for MLMD.
引用
收藏
页码:473 / 481
页数:9
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