Bio-inspired in situ crosslinking and mineralization of electrospun collagen scaffolds for bone tissue engineering

被引：156

作者：

Dhand, Chetna ^{[1
]}

Ong, Seow Theng ^{[2
]}

Dwivedi, Neeraj ^{[3
]}

Diaz, Silvia Marrero ^{[4
,5
]}

Venugopal, Jayarama Reddy ^{[4
]}

Navaneethan, Balchandar ^{[4
]}

Fazil, Mobashar H. U. T. ^{[2
]}

Liu, Shouping ^{[1
,6
]}

Seitz, Vera ^{[5
]}

Wintermantel, Erich ^{[5
]}

Beuerman, Roger W. ^{[1
,6
]}

Ramakrishna, Seeram ^{[4
,7
]}

Verma, Navin K. ^{[2
]}

Lakshminarayanan, Rajamani ^{[1
,6
]}

机构：

[1] The Academia, Singapore Eye Res Inst, Antiinfect Res Grp, 20 Coll Rd,Discovery Tower, Singapore 169856, Singapore

[2] Nanyang Technol Univ, Lee Kong Chian Sch Med, Expt Med Bldg, Singapore 636921, Singapore

[3] Natl Univ Singapore, Dept Elect & Comp Engn, 3 Engn Dr 3, Singapore 117583, Singapore

[4] Natl Univ Singapore, Dept Mech Engn, Ctr Nanofibers & Nanotechnol, Singapore 119260, Singapore

[5] Tech Univ Munich, Inst Med & Polymer Engn, Boltzmannstr 15, D-85748 Garching, Germany

[6] Duke NUS Grad Med Sch, Ophthalmol & Visual Sci Acad Clin Program, Singapore 169857, Singapore

[7] Jinan Univ, Guangdong Hongkong Macau Inst CNS Regenerat GHMIC, Guangzhou 510632, Guangdong, Peoples R China

来源：

BIOMATERIALS | 2016年 / 104卷

基金：

英国医学研究理事会;

关键词：

Collagen; Catecholamine; Crosslinking; Bone; Electrospinning; Mineralization; NANOCOMPOSITE NANOFIBERS; SURFACE-CHEMISTRY; STEM-CELLS; REGENERATION; GELATIN; FIBERS; FRACTURES; DOPAMINE; WATER; PHOTOLUMINESCENT;

D O I：

10.1016/j.biomaterials.2016.07.007

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Bone disorders are the most common cause of severe long term pain and physical disability, and affect millions of people around the world. In the present study, we report bio-inspired preparation of bone like composite structures by electrospinning of collagen containing catecholamines and Ca2+. The presence of divalent cation induces simultaneous partial oxidative polymerization of catecholamines and crosslinking of collagen nanofibers, thus producing mats that are mechanically robust and confer photoluminescence properties. Subsequent mineralization of the mats by ammonium carbonate leads to complete oxidative polymerization of catecholamines and precipitation of amorphous CaCO3. The collagen composite scaffolds display outstanding mechanical properties with Young's modulus approaching the limits of cancellous bone. Biological studies demonstrate that human fetal osteoblasts seeded on to the composite scaffolds display enhanced cell adhesion, penetration, proliferation, differentiation and osteogenic expression of osteocalcin, osteopontin and bone matrix protein when compared to pristine collagen or tissue culture plates. Among the two catecholamines, mats containing norepinephrine displayed superior mechanical, photoluminescence and biological properties than mats loaded with dopamine. These smart multifunctional scaffolds could potentially be utilized to repair and regenerate bone defects and injuries. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：323 / 338

页数：16

共 53 条

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