Fabrication of a Structure-Specific RNA Binder for Array Detection of Label-Free MicroRNA

被引：78

作者：

Lee, Jeong Min ^{[1
,2
]}

Cho, Hyunmin ^{[1
,2
]}

Jung, Yongwon ^{[1
,2
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, BioNanotechnol Res Ctr, Taejon 305600, South Korea

[2] Univ Sci & Technol, Sch Engn, Taejon 305333, South Korea

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 46期

关键词：

biosensors; microarrays; microRNA; protein design; RNA recognition; EXPRESSION; MICROARRAYS; CANCER; INTERFERENCE; RECOGNITION; BIOGENESIS; LNA;

D O I：

10.1002/anie.201004000

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Like an antibody: A novel structure-specific RNA-binding protein was designed to stably and specifically bind to surface-bound microRNAs. By acting like an antibody, this RNA binder enabled the universal detection of hybridized microRNAs on array surfaces (see picture) without any enzymatic amplification or labeling reactions. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：8662 / 8665

页数：4

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