Synchrotron X-ray biosample imaging: opportunities and challenges

被引：3

作者：

Sena, Gabriela ^{[1
,2
]}

Fidalgo, Gabriel ^{[1
]}

Paiva, Katrine ^{[1
]}

Barcelos, Renan ^{[1
,2
]}

Nogueira, Liebert Parreiras ^{[3
]}

Colaco, Marcos Vinicius ^{[1
]}

Gonzalez, Marcelo Salabert ^{[4
]}

Azambuja, Patricia ^{[4
]}

Colaco, Gustavo ^{[5
]}

da Silva, Helio Ricardo ^{[5
]}

de Moura Meneses, Anderson Alvarenga ^{[6
]}

Barroso, Regina Cely ^{[1
]}

机构：

[1] Univ Estado Rio De Janeiro, Phys Inst, Lab Appl Phys Biomed Sci, Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, COPPE, Rio De Janeiro, Brazil

[3] Univ Oslo, Inst Clin Dent, Oral Res Lab ORL, Oslo, Norway

[4] Fed Univ Fluminense, Postgrad Program Sci & Biotechnol, Rio De Janeiro, Brazil

[5] Univ Fed Rural Rio de Janeiro, Lab Herpetol, Rio De Janeiro, Brazil

[6] Fed Univ Western, Lab Computat Intelligence, Santarem, Para, Brazil

来源：

BIOPHYSICAL REVIEWS | 2022年 / 14卷 / 03期

关键词：

MICRO-CT; PHASE; MICROTOMOGRAPHY; MORPHOLOGY; BIOMATERIALS; COMPETITION; TOMOGRAPHY; INCLUSIONS; PREDATION; RADIATION;

D O I：

10.1007/s12551-022-00964-4

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Synchrotron radiation phase-contrast microtomography is sensitive to low attenuating tissues, giving an alternative visualisation of the sample and being useful for investigating microstructure inside biological specimens without staining them with a contrast medium. The phase-contrast technique has been widely used in the scientific community, as it is a technique associated with radiography and microscopy and able to enhance contrast in soft tissues, specifically at the edges, showing details that could not be seen by the absorption technique. This work aims to show the ability of synchrotron-based phase-contrast microtomography for the visualisation of soft tissues and hard internal structures of millimetre-sized biological organisms. Case studies of the anatomy of Rhodnius prolixus head and Thoropa miliaris tadpole are presented to illustrate the imaging technique.

引用

页码：625 / 633

页数：9

共 64 条

[1] Elastic transformation of histological slices allows precise co-registration with microCT data sets for a refined virtual histology approach [J].

Albers, Jonas ;

Svetlove, Angelika ;

Alves, Justus ;

Kraupner, Alexander ;

di Lillo, Francesca ;

Markus, M. Andrea ;

Tromba, Giuliana ;

Alves, Frauke ;

Dullin, Christian .

SCIENTIFIC REPORTS, 2021, 11 (01)

[2] Phase contrast X-ray microtomography of the Rhodnius prolixus head: Comparison of direct reconstruction and phase retrieval approach [J].

Almeida, A. P. ;

Braz, D. ;

Nogueira, L. P. ;

Colaco, M. V. ;

Soares, J. ;

Cardoso, S. C. ;

Garcia, E. S. ;

Azambuja, P. ;

Gonzalez, M. S. ;

Mohammadi, S. ;

Tromba, G. ;

Barroso, R. C. .

RADIATION PHYSICS AND CHEMISTRY, 2014, 95 :243-246

[3] Application of the SR-PhC-μCT technique with phase retrieval for the characterization of internal and external structures of Rhodnius prolixus [J].

Almeida, A. P. ;

Braz, D. ;

Nogueira, L. P. ;

Colaco, M. V. ;

Soares, J. ;

Cardoso, S. C. ;

Garcia, E. S. ;

Azambuja, P. ;

Gonzalez, M. S. ;

Mohammadi, S. ;

Tromba, G. ;

Barroso, R. C. .

JOURNAL OF INSTRUMENTATION, 2013, 8

[4] Imaging challenges in biomaterials and tissue engineering [J].

Appel, Alyssa A. ;

Anastasio, Mark A. ;

Larson, Jeffery C. ;

Brey, Eric M. .

BIOMATERIALS, 2013, 34 (28) :6615-6630

[5] Ornithodoros faccinii n. sp (Acari: Ixodida: Argasidae) parasitizing the frog Thoropa miliaris (Amphibia: Anura: Cycloramphidae) in Brazil [J].

Barros-Battesti, Darci Moraes ;

Landulfo, Gabriel Alves ;

Luz, Hermes Ribeiro ;

Marcili, Arlei ;

Onofrio, Valeria Castilho ;

Famadas, Katia Maria .

PARASITES & VECTORS, 2015, 8

[6] Interface-specific x-ray phase retrieval tomography of complex biological organs [J].

Beltran, M. A. ;

Paganin, D. M. ;

Siu, K. K. W. ;

Fouras, A. ;

Hooper, S. B. ;

Reser, D. H. ;

Kitchen, M. J. .

PHYSICS IN MEDICINE AND BIOLOGY, 2011, 56 (23) :7353-7369

[7] Imaging applications of synchrotron X-ray phase-contrast microtomography in biological morphology and biomaterials science. 1. General aspects of the technique and its advantages in the analysis of millimetre-sized arthropod structure [J].

Betz, Oliver ;

Wegst, Ulrike ;

Weide, Daniela ;

Heethoff, Michael ;

Helfen, Lukas ;

Lee, Wah-Keat ;

Cloetens, Peter .

JOURNAL OF MICROSCOPY, 2007, 227 (01) :51-71

[8] X-ray phase-contrast imaging: from pre-clinical applications towards clinics [J].

Bravin, Alberto ;

Coan, Paola ;

Suortti, Pekka .

PHYSICS IN MEDICINE AND BIOLOGY, 2013, 58 (01) :R1-R35

[9] Boosting Resolution and Recovering Texture of 2D and 3D Micro-CT Images with Deep Learning [J].

Da Wang, Ying ;

Armstrong, Ryan T. ;

Mostaghimi, Peyman .

WATER RESOURCES RESEARCH, 2020, 56 (01)

[10] Phase contrast X-ray synchrotron imaging for assessing external and internal morphology of Rhodnius prolixus [J].

de Almeida, Andre P. ;

Soares, Jose ;

Meneses, Anderson A. M. ;

Cardoso, Simone C. ;

Braz, Delson ;

Garcia, Eloi S. ;

Gonzalez, Marcelo S. ;

Azambuja, Patricia ;

Barroso, Regina C. .

APPLIED RADIATION AND ISOTOPES, 2012, 70 (07) :1340-1343

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