An ex vivo model using human peritoneum to explore mesh-tissue integration

被引：5

作者：

Falk, Peter ^{[1
]}

Ruiz-Jasbon, Fernando ^{[1
,2
]}

Strigard, Karin ^{[3
]}

Gunnarsson, Ulf ^{[3
]}

Ivarsson, Marie-Lois ^{[1
]}

机构：

[1] Univ Gothenburg, Inst Clin Sci, Dept Surg, Sahlgrenska Acad, SE-41685 Gothenburg, Sweden

[2] Hallands Hosp, Dept Surg, SE-43480 Kungsbacka, Sweden

[3] Umea Univ, Dept Surg & Perioperat Sci, S-90185 Umea, Sweden

来源：

BIOLOGY OPEN | 2017年 / 6卷 / 09期

关键词：

Experimental model; Peritoneum; Peritoneal tissue; Mesh; Synthetic mesh; Biocompatibility; MESOTHELIAL CELLS; SALINE;

D O I：

10.1242/bio.024992

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Biological compatibility, in terms of implantation of foreign mesh material in hernia surgery, still needs experimental investigation. The present study develops an experimental model using human peritoneum to study the integration between tissue and different mesh material. The ex vivo model using peritoneal tissue was studied with different mesh material, and integration was monitored over time using microscopy. The peritoneal model could be kept viable in culture for several weeks. Cell migration was seen after 7-10 days in culture and could be further monitored over several weeks. The use of a human artificial model environment enabling the investigation of tissue/mesh integration has, to our knowledge, not been described previously. This proof-of-concept model was developed for the investigation of peritoneal biology and the integration between tissue and different mesh material. It has the potential to be useful in studies on other important biological mechanisms involving the peritoneum.

引用

页码：1391 / 1395

页数：5

共 20 条

[1] Investigation of Tumor-Peritoneal Interactions in the Pathogenesis of Peritoneal Metastases using a Novel Ex Vivo Peritoneal Model [J].