Haemophilus parasuis infection activates chemokine RANTES in PK-15 cells

被引：19

作者：

Chen, Yushan ^{[1
]}

Zhou, Shanshan ^{[1
]}

Hua, Kexin ^{[1
]}

Xiao, Hongde ^{[2
]}

Li, Zili ^{[1
]}

Liu, Mei ^{[1
]}

Luo, Rui ^{[1
]}

Bi, Dingren ^{[1
]}

Zhou, Rui ^{[1
]}

Jin, Hui ^{[1
]}

机构：

[1] Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

[2] Hubei Ctr Anim Dis Control & Prevent, Wuhan 430070, Peoples R China

来源：

MOLECULAR IMMUNOLOGY | 2015年 / 67卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Haemophilus parasuis; RANTES; NF-kappa B; TLR pathways; NF-KAPPA-B; ALVEOLAR EPITHELIAL-CELLS; SIGNALING PATHWAYS; PROMOTER ACTIVATION; MAP KINASE; INHIBITION; EXPRESSION; RECEPTORS; CCR5;

D O I：

10.1016/j.molimm.2015.06.029

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RANTES is a member of the CC chemokine involved in inflammation and immune response during pathogen infection. In our previous study, Haemophilus parasuis (H. parasuis), which is responsible for the great economic losses in the pig industry worldwide, has been shown to enhance RANTES expression in PK-15 cells. However, the mechanisms behind this biological phenomenon have remained unclear. In this study, we showed that H. parasuis infection significantly upregulated RANTES gene transcription in a time- and dose-dependent manner. Promoter analysis by site-directed mutagenesis indicated that the nuclear factor NF-kappa B binding site was the most important cis-regulatory element controlling H. parasuis-induced RANTES transcription. Inhibition of NF-kappa B and JNK activity also significantly reduced H. parasuis-induced RANTES production. In addition, TLRs signaling pathway was found to be involved in H. parasuis induced-RANTES expression. These results represent an important molecular mechanism whereby H. parasuis induced RANTES in the inflammatory response. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：661 / 666

页数：6

共 30 条

[1]

Algarté M, 1999, J VIROL, V73, P2694

[2] Analysis of functional domains of interferon regulatory factor 7 and its association with IRF-3 [J].

Au, WC ;

Yeow, WS ;

Pitha, PM .

VIROLOGY, 2001, 280 (02) :273-282

[3] Distinct intracellular signaling pathways control the synthesis of IL-8 and RANTES in TLR1/TLR2, TLR3 or NOD1 activated human airway epithelial cells [J].

Berube, Julie ;

Bourdon, Celine ;

Yao, Yu ;

Rousseau, Simon .

CELLULAR SIGNALLING, 2009, 21 (03) :448-456

[4] TARF6 is a signal transducer for interleukin-1 [J].

Cao, ZD ;

Xiong, J ;

Takeuchi, M ;

Kurama, T ;

Goeddel, DV .

NATURE, 1996, 383 (6599) :443-446

[5] Regulation of RANTES promoter activation in alveolar epithelial cells after cytokine stimulation [J].

Casola, A ;

Henderson, A ;

Liu, TH ;

Garofalo, RP ;

Brasier, AR .

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2002, 283 (06) :L1280-L1290

[6] Multiple cis regulatory elements control RANTES promoter activity in alveolar epithelial cells infected with respiratory syncytial virus [J].

Casola, A ;

Garofalo, RP ;

Haeberle, H ;

Elliott, TF ;

Lin, RT ;

Jamaluddin, M ;

Brasier, AR .

JOURNAL OF VIROLOGY, 2001, 75 (14) :6428-6439

[7] Haemophilus parasuis induces activation of NF-κB and MAP kinase signaling pathways mediated by toll-like receptors [J].

Chen, Yushan ;

Liu, Ting ;

Langford, Paul ;

Hua, Kexin ;

Zhou, Shanshan ;

Zhai, Yajun ;

Xiao, Hongde ;

Luo, Rui ;

Bi, Dingren ;

Jin, Hui ;

Zhou, Rui .

MOLECULAR IMMUNOLOGY, 2015, 65 (02) :360-366

[8] Haemophilus parasuis infection activates the NF-κB pathway in PK-15 cells through IκB degradation [J].

Chen, Yushan ;

Jin, Hui ;

Chen, Ping ;

Li, Zili ;

Meng, Xianrong ;

Liu, Mei ;

Li, Shaowen ;

Shi, Deshi ;

Xiao, Yuncai ;

Wang, Xiliang ;

Zhou, Zutao ;

Bi, Dingren ;

Zhou, Rui .

VETERINARY MICROBIOLOGY, 2012, 160 (1-2) :259-263

[9] Neutralization of macrophage inflammatory protein 2 (MIP-2) and MIP-1α attenuates neutrophil recruitment in the central nervous system during experimental bacterial meningitis [J].

Diab, A ;

Abdalla, H ;

Li, HL ;

Shi, FD ;

Zhu, J ;

Höjberg, B ;

Lindquist, L ;

Wretlind, B ;

Bakhiet, M ;

Link, H .

INFECTION AND IMMUNITY, 1999, 67 (05) :2590-2601

[10] CCR5 Is Involved in Resolution of Inflammation in Proteoglycan-Induced Arthritis [J].

Doodes, Paul D. ;

Cao, Yanxia ;

Hamel, Keith M. ;

Wang, Yumei ;

Rodeghero, Rachel L. ;

Kobezda, Tamas ;

Finnegan, Alison .

ARTHRITIS AND RHEUMATISM, 2009, 60 (10) :2945-2953

← 1 2 3 →