Overlapping Local and Long-Range RNA-RNA Interactions Modulate Dengue Virus Genome Cyclization and Replication

被引：65

作者：

de Borba, Luana ^{[1
]}

Villordo, Sergio M. ^{[1
]}

Iglesias, Nestor G. ^{[1
]}

Filomatori, Claudia V. ^{[1
]}

Gebhard, Leopoldo G. ^{[1
]}

Gamarnik, Andrea V. ^{[1
]}

机构：

[1] Consejo Nacl Invest Cient & Tecn, Fdn Inst Leloir, RA-1033 Buenos Aires, DF, Argentina

来源：

JOURNAL OF VIROLOGY | 2015年 / 89卷 / 06期

关键词：

VIRAL-RNA; 3'-NONCODING REGION; ELEMENT; TRANSLATION; PROTEINS; PREDICTION; MOSQUITO; REVEALS; MOTIFS; SYSTEM;

D O I：

10.1128/JVI.02677-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The dengue virus genome is a dynamic molecule that adopts different conformations in the infected cell. Here, using RNA folding predictions, chemical probing analysis, RNA binding assays, and functional studies, we identified new cis-acting elements present in the capsid coding sequence that facilitate cyclization of the viral RNA by hybridization with a sequence involved in a local dumbbell structure at the viral 3' untranslated region (UTR). The identified interaction differentially enhances viral replication in mosquito and mammalian cells.

引用

收藏

页码：3430 / 3437

页数：8

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