Isotopic labelling of the polypeptide subunits of the isolated photosystem II reaction-centre complex of Chlamydomonas reinhardtii suggests an αβ heterodimeric structure for cytochrome b-559

被引：0

作者：

Alizadeh, S ^{[1
]}

Morais, F ^{[1
]}

Barber, J ^{[1
]}

Nixon, PJ ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AY, England

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 1999年 / 48卷 / 2-3期

关键词：

cytochrome b; photosystem II; reaction-centre complexes;

D O I：

10.1016/S1011-1344(99)00041-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The photosystem II reaction-centre (PSII RC) complex contains live polypeptides: D1, D2, PsbI and the alpha and beta subunits of cytochrome b-559. In order to determine their stoichiometry, we have isolated a PSII RC from a mutant of the green alga Chlamydomonas reinhardtii, lacking the photosystem I and chloroplast ATP-ase complexes, which has been grown in the presence of [C-14] -acetate. The stoichiometry of the polypeptides is estimated by SDS-polyacrylamide electrophoresis followed by phosphorimage analysis of the gel. Our data suggest equimolar amounts of each polypeptide. Assuming the presence of homogeneous PSII RC complexes, our results are consistent with an alpha beta heterodimeric structure for cytochrome b-559. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：148 / 153

页数：6

共 32 条

[21] A NUCLEAR-ENCODED SUBUNIT OF THE PHOTOSYSTEM-II REACTION-CENTER [J].

IRRGANG, KD ;

SHI, LX ;

FUNK, C ;

SCHRODER, WP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (29) :17588-17593

[22]

KUNSTNER P, 1995, J BIOL CHEM, V270, P9651

[23] Structural model of cytochrome b(559) in photosystem II based on a mutant with genetically fused subunits [J].

McNamara, VP ;

Sutterwala, FS ;

Pakrasi, HB ;

Whitmarsh, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (25) :14173-14178

[24] STOICHIOMETRY OF CYTOCHROME B-559 IN PHOTOSYSTEM-II [J].

MIYAZAKI, A ;

SHINA, T ;

TOYOSHIMA, Y ;

GOUNARIS, K ;

BARBER, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 975 (01) :142-147

[25] The chloroplast-encoded α subunit of cytochrome b-559 is required for assembly of the photosystem two complex in both the light and the dark in Chlamydomonas reinhardtii [J].

Morais, F ;

Barber, J ;

Nixon, PJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (45) :29315-29320

[26] ISOLATION OF A PHOTOSYSTEM-II REACTION CENTER CONSISTING OF D-1 AND D-2 POLYPEPTIDES AND CYTOCHROME-B-559 [J].

NANBA, O ;

SATOH, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (01) :109-112

[27] EPR STUDY OF CYTOCHROME IN THE D1D2 CYT B-559 COMPLEX [J].

SHUVALOV, VA ;

FIEGE, R ;

SCHREIBER, U ;

LENDZIAN, F ;

LUBITZ, W .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1995, 1228 (2-3) :175-180

[28] SPECTRAL AND THERMODYNAMIC PROPERTIES OF THE 2 HEMES OF THE D1D2CYTOCHROME B-559 COMPLEX OF SPINACH [J].

SHUVALOV, VA ;

SCHREIBER, U ;

HEBER, U .

FEBS LETTERS, 1994, 337 (03) :226-230

[29] LOW-TEMPERATURE PHOTOCHEMISTRY AND SPECTRAL PROPERTIES OF A PHOTOSYSTEM-2 REACTION CENTER COMPLEX CONTAINING THE PROTEIN-D1 AND PROTEIN-D2 AND 2 HEMES OF CYT-B-559 [J].

SHUVALOV, VA ;

HEBER, U ;

SCHREIBER, U .

FEBS LETTERS, 1989, 258 (01) :27-31

[30]

Whithmarsh J, 1996, ADV PHOTOSYNTH, V4, P249

← 1 2 3 4 →