The genetic screening of preimplantation embryos by comparative genomic hybridisation

被引:0
作者
Traversa, Maria V. [1 ]
Marshall, James [1 ]
McArthur, Steven [1 ]
Leigh, Don [1 ]
机构
[1] Genea, Sydney, NSW 2000, Australia
关键词
aneuploidy; comparative genomic hybridization; PGD; blastocyst; BLASTOCYST STAGE; DIAGNOSIS;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Comparative genomic hybridization (CGH) is an indirect DNA-based test which allows for the accurate analysis of aneuploidy involving any of the 24 types of chromosomes present (22 autosomes and the X and Y sex chromosomes). Traditionally, embryos have been screened using fluorescence in situ hybridization (FISH) - a technique that was limited in the number of chromosomes able to be identified in any one sample. Early CGH reports on aneuploidy in preimplantation embryos showed that any of the 24 chromosomes could be involved and so FISH methods were going to be ineffective in screening out abnormal embryos. Our results from routine clinical application of array CGH in preimplantation genetic diagnosis (PGD) patients confirm previous reports on patterns of chromosomal contribution to aneuploidy. The pregnancy outcomes following embryo transfer also indicate that despite the requirement to freeze embryos, rates are encouraging, and successful ongoing pregnancies can be achieved. Reproductive Biology 2011 Suppl. 3: 51-60.
引用
收藏
页码:51 / 60
页数:10
相关论文
共 8 条
  • [1] Moving to blastocyst biopsy for preimplantation genetic diagnosis and single embryo transfer at Sydney IVF
    de Boer, KA
    Catt, JW
    Jansen, RPS
    Leigh, D
    McArthur, S
    [J]. FERTILITY AND STERILITY, 2004, 82 (02) : 295 - 298
  • [2] Comprehensive molecular cytogenetic analysis of the human blastocyst stage
    Fragouli, E.
    Lenzi, M.
    Ross, R.
    Katz-Jaffe, M.
    Schoolcraft, W. B.
    Wells, D.
    [J]. HUMAN REPRODUCTION, 2008, 23 (11) : 2596 - 2608
  • [3] Comprehensive chromosome screening of polar bodies and blastocysts from couples experiencing repeated implantation failure
    Fragouli, Elpida
    Katz-Jaffe, Mandy
    Alfarawati, Samer
    Stevens, John
    Colls, Pere
    Goodall, N-neka
    Tormasi, Sophia
    Gutierrez-Mateo, Cristina
    Prates, Renata
    Schoolcraft, William B.
    Munne, Santiago
    Wells, Dagan
    [J]. FERTILITY AND STERILITY, 2010, 94 (03) : 875 - 887
  • [4] Aneuploidy in the miscarriages of infertile women and the potential benefit of preimplanation genetic diagnosis
    Lathi, Ruth B.
    Westphal, Lynn M.
    Milki, Amin A.
    [J]. FERTILITY AND STERILITY, 2008, 89 (02) : 353 - 357
  • [5] Improved detection of aneuploid blastocysts using a new 12-chromosome FISH test
    Munne, S.
    Fragouli, E.
    Colls, P.
    Katz-Jaffe, M. G.
    Schoolcraft, W. B.
    Wells, D.
    [J]. REPRODUCTIVE BIOMEDICINE ONLINE, 2010, 20 (01) : 92 - 97
  • [6] Technology requirements for preimplantation genetic diagnosis to improve assisted reproduction outcomes
    Munne, Santiago
    Wells, Dagan
    Cohen, Jacques
    [J]. FERTILITY AND STERILITY, 2010, 94 (02) : 408 - 430
  • [7] Clinical application of comprehensive chromosomal screening at the blastocyst stage
    Schoolcraft, William B.
    Fragouli, Elpida
    Stevens, John
    Munne, Santiago
    Katz-Jaffe, Mandy G.
    Wells, Dagan
    [J]. FERTILITY AND STERILITY, 2010, 94 (05) : 1700 - 1706
  • [8] A molecular strategy for routine preimplantation genetic diagnosis in both reciprocal and Robertsonian translocation carriers
    Traversa, M. V.
    Carey, L.
    Leigh, D.
    [J]. MOLECULAR HUMAN REPRODUCTION, 2010, 16 (05) : 329 - 337