Clear distinction between Burkholderia mallei and Burkholderia pseudomallei using fluorescent motB primers

被引：4

作者：

Schmoock, Gernot ^{[1
]}

Elschner, Mandy ^{[1
,2
]}

Sprague, Lisa D. ^{[1
]}

机构：

[1] Bundesforsch Inst Tiergesundheit, Friedrich Loeffler Inst, DE-07743 Jena, Germany

[2] Bundesforsch Inst Tiergesundheit, Friedrich Loeffler Inst, Natl & OIE Reference Lab Glanders, DE-07743 Jena, Germany

来源：

ACTA VETERINARIA SCANDINAVICA | 2015年 / 57卷

关键词：

Duplex PCR; Fluorescent primers; Burkholderia;

D O I：

10.1186/s13028-015-0104-4

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Background: A frame-shift mutation in the flagellum motor gene motB coding for the chemotaxis MotB protein of Burkholderia mallei has been utilized to design a conventional duplex PCR assay with fluorescent labelled primers. Findings: Species specificity was tested with a panel of 13 Burkholderia type strains. A total of 41 B. mallei field strains, 36 B. pseudomallei field strains, and 1 B. thailandensis field strain from different geographic regions were tested and correctly identified. Testing of 55 non-Burkholderia bacterial species revealed 100% specificity of the assay. The minimum detection limit was 1 pg DNA or 160 GE for B. mallei and 130 GE for B. pseudomallei, respectively. Conclusions: This assay enables the clear distinction between B. mallei and B. pseudomallei/B. thailandensis.

引用

页数：4

共 12 条

[1] Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species [J].

Brett, PJ ;

DeShazer, D ;

Woods, DE .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 :317-320

[2]

Cheng AC, 2005, EUROSURVEILLANCE, V10, P3

[3] Zoonosis update - Glanders [J].

Dvorak, Glenda D. ;

Spickler, Anna R. .

JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION, 2008, 233 (04) :570-577

[4]

Gilad Jacob, 2007, Recent Pat Antiinfect Drug Discov, V2, P233, DOI 10.2174/157489107782497335

[5] PCR-based Methodologies Used to Detect and Differentiate the Burkholderia pseudomallei complex: B. pseudomallei, B. mallei, and B. thailandensis [J].

Lowe, Woan ;

March, Jordon K. ;

Bunnell, Annette J. ;

O'Neill, Kim L. ;

Robison, Richard A. .

CURRENT ISSUES IN MOLECULAR BIOLOGY, 2014, 16 :23-54

[6] Public health assessment of potential biological terrorism agents [J].

Rotz, LD ;

Khan, AS ;

Lillibridge, SR ;

Ostroff, SM ;

Hughes, JM .

EMERGING INFECTIOUS DISEASES, 2002, 8 (02) :225-230

[7] DNA microarray-based detection and identification of Burkholderia mallei, Burkholderia pseudomallei and Burkholderia spp. [J].

Schmoock, Gernot ;

Ehricht, Ralf ;

Melzer, Falk ;

Rassbach, Astrid ;

Scholz, Holger C. ;

Neubauer, Heinrich ;

Sachse, Konrad ;

Mota, Rinaldo Aparecido ;

Saqib, Muhammad ;

Elschner, Mandy .

MOLECULAR AND CELLULAR PROBES, 2009, 23 (3-4) :178-187

[8] Detection of the reemerging agent Burkholderia mallei in a recent outbreak of glanders in the United Arab Emirates by a newly developed fliP-based polymerase chain reaction assay [J].

Scholz, HC ;

Joseph, M ;

Tomaso, H ;

Al Dahouk, S ;

Witte, A ;

Kinne, J ;

Hagen, RM ;

Wernery, R ;

Wernery, U ;

Neubauer, H .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2006, 54 (04) :241-247

[9] Antibiotic susceptibility of 65 isolates of Burkholderia pseudomallei and Burkholderia mallei to 35 antimicrobial agents [J].

Thibault, FM ;

Hernandez, E ;

Vidal, DR ;

Girardet, M ;

Cavallo, JD .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2004, 54 (06) :1134-1138

[10] Development of 5′ nuclease real-time PCR assays for the rapid identification of the Burkholderia mallei//Burkholderia pseudomallei complex [J].

Tomaso, H ;

Scholz, HC ;

Al Dahouk, S ;

Pitt, TL ;

Treu, TM ;

Neubauer, H .

DIAGNOSTIC MOLECULAR PATHOLOGY, 2004, 13 (04) :247-253

← 1 2 →