Photothermal ablation of amyloid aggregates by gold nanoparticles

被引:55
|
作者
Triulzi, Robert C. [2 ]
Dai, Qiu [1 ,3 ]
Zou, Jianhua [1 ,3 ]
Leblanc, Roger M. [2 ]
Gu, Qun [4 ]
Orbulescu, Jhony [2 ]
Huo, Qun [1 ,3 ]
机构
[1] Univ Cent Florida, Dept Chem, Nanosci Technol Ctr, Orlando, FL 32826 USA
[2] Univ Miami, Dept Chem, Coral Gables, FL 33146 USA
[3] Univ Cent Florida, Dept Mech Mat & Aerosp Engn, Nanosci Technol Ctr, Orlando, FL 32826 USA
[4] Pacific Nanotechnol, Santa Clara, CA 95054 USA
基金
美国国家科学基金会;
关键词
Au nanoparticle; amyloid; aggregation; monofunctionalization; photothermal; ablation;
D O I
10.1016/j.colsurfb.2007.12.006
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Amyloid peptide (A beta) is found in the brain and blood of both healthy and diseased individuals alike. However, upon secondary structure transformation to a beta-sheet dominated conformation, the protein aggregates. These aggregates accumulate to form neuritic plaques that are implicated in the pathogenesis of Alzheimer's disease. Gold nanoparticles are excellent photon-thermal energy converters. The extinction coefficient of the surface plasmon band of gold nanoparticles is very large when compared to typical organic dyes. In this study, gold nanoparticle-A beta conjugates were prepared and the photothermal ablation of amyloid peptide aggregates by laser irradiation was studied. Monofunctional gold nanoparticles were prepared using a recently reported solid phase modification method and then coupled to fragments of A beta peptide, namely A beta(31-35) and A beta(25-35). The conjugates were then mixed with A beta fragments in solution. The aggregated peptide formation was studied by a series of spectroscopic and microscopic techniques. The peptide aggregates were then irradiated by a continuous laser. With gold nanoparticle-A beta conjugates present the aggregates were destroyed by photothermal ablation. Gold nanoparticles without A beta conjugation were not incorporated into the aggregates and when irradiated did not result in photothermal ablation. With gold nanoparticle-A beta conjugates the ablation was selective to the site of irradiation and minimal damage was observed as a result of thermal diffusion. In addition to the application of photoablation to a protein-based sample the nanoparticles and the chemistry involved provide an easily monofunctionalized photothermal material for the biological conjugation. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:200 / 208
页数:9
相关论文
共 50 条
  • [21] Bioproduction of gold nanoparticles for photothermal therapy
    Silva, Catarina Oliveira
    Rijo, Patricia
    Molpeceres, Jesus
    Ascensao, Lia
    Roberto, Amilcar
    Fernandes, Ana Sofia
    Gomes, Ricardo
    Pinto Coelho, Joao M.
    Gabriel, Ana
    Vieira, Pedro
    Reis, Catarina Pinto
    THERAPEUTIC DELIVERY, 2016, 7 (05) : 287 - 304
  • [22] Gold Nanoparticles for Photothermal and Photodynamic Therapy
    Broadbent, Matthew
    Chadwick, Samantha J.
    Brust, Mathias
    Volk, Martin
    ACS OMEGA, 2024, 9 (44): : 44846 - 44859
  • [23] Photothermal heterodyne holography of gold nanoparticles
    Absil, E.
    Tessier, G.
    Gross, M.
    Atlan, M.
    Warnasooriya, N.
    Suck, S.
    Coppey-Moisan, M.
    Fournier, D.
    OPTICS EXPRESS, 2010, 18 (02): : 780 - 786
  • [24] Gold Nanoparticles for Photothermal Cancer Therapy
    Vines, Jeremy B.
    Yoon, Jee-Hyun
    Ryu, Na-Eun
    Lim, Dong-Jin
    Park, Hansoo
    FRONTIERS IN CHEMISTRY, 2019, 7
  • [25] Spherical aggregates composed of gold nanoparticles
    Chen, Chi-Chang
    Kuo, Ping-Lin
    Cheng, Yu-Chen
    NANOTECHNOLOGY, 2009, 20 (05)
  • [26] Photothermal effects in small gold nanorod aggregates for therapeutic applications
    Pratap, Dheeraj
    Shah, Ram Krishna
    Khandekar, Sameer
    Soni, Sanjeev
    APPLIED NANOSCIENCE, 2022, 12 (07) : 2045 - 2058
  • [27] Photothermal effects in small gold nanorod aggregates for therapeutic applications
    Dheeraj Pratap
    Ram Krishna Shah
    Sameer Khandekar
    Sanjeev Soni
    Applied Nanoscience, 2022, 12 : 2045 - 2058
  • [28] Plasmonically Modulated Gold Nanostructures for Photothermal Ablation of Bacteria
    Guan, Guijian
    Win, Khin Yin
    Yao, Xiang
    Yang, Wensheng
    Han, Ming-Yong
    ADVANCED HEALTHCARE MATERIALS, 2021, 10 (03)
  • [29] NOVEL GOLD SPECKLED SILICA NANOPARTICLES AS MEDIATORS OF TUMOR IMAGING AND PHOTOTHERMAL ABLATION THERAPY FOR BREAST CANCER
    Iwakuma, N.
    Toh, U.
    Shirouzu, K.
    Sharma, P.
    Moudgil, B. M.
    Grobmyer, S. R.
    ANNALS OF ONCOLOGY, 2010, 21 : 44 - 44
  • [30] In vivo photothermal tumour ablation using gold nanorods
    de Freitas, L. F.
    Zanelatto, L. C.
    Mantovani, M. S.
    Silva, P. B. G.
    Ceccini, R.
    Grecco, C.
    Moriyama, L. T.
    Kurachi, C.
    Martins, V. C. A.
    Plepis, A. M. G.
    LASER PHYSICS, 2013, 23 (06)