H-1 NMR studies of the Fe7S8 ferredoxin from Bacillus schlegelii: A further attempt to understand Fe3S4 clusters

被引:24
作者
Aono, S
Bertini, I
Cowan, JA
Luchinat, C
Rosato, A
Viezzoli, MS
机构
[1] UNIV FLORENCE, DEPT CHEM, I-50121 FLORENCE, ITALY
[2] OHIO STATE UNIV, DEPT CHEM, COLUMBUS, OH 43210 USA
[3] UNIV BOLOGNA, INST AGR CHEM, I-40126 BOLOGNA, ITALY
来源
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY | 1996年 / 1卷 / 06期
关键词
ferredoxin; iron-sulfur clusters; NMR; hyperfine shifts; magnetic interactions;
D O I
10.1007/s007750050087
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The oxidized Fe7S8 ferredoxin from Bacillus schlegelii, containing both [Fe3S4](+) and [Fe4S4](2+) clusters, has been investigated by H-1 NMR spectroscopy. An extensive sequence-specific assignment of the hyperfine-shifted resonances has been obtained by making use of a computer-generated structural model. The pattern and the temperature dependence of the hyperfine shifts of the beta-CH2 protons of the cysteines coordinating the [Fe3S4](+) cluster are rationalized in terms of magnetic interactions between the iron ions. The same approach holds for the hyperfine coupling with Fe-57. It is shown that the magnetic interactions are more asymmetric in Fe7S8 ferredoxins than in Fe3S4 ferredoxins. The NMR non-observability of the beta-CH2 protons of coordinated cysteines in the one-electron-reduced form has been discussed.
引用
收藏
页码:523 / 528
页数:6
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