In this study, the influences of fiber blend ratio on quality of cotton/polyester and cotton/acrylic rotor spun yams, which have wide range usage area in textile, were investigated. For this purpose, cotton fibers grown in Sanhurfa region, polyester fibers, and acrylic fibers were used as raw materials. Ne 20/1 Cotton/Polyester yams in four different blend ratios and Ne 20/1 cotton/acrylic yams in four different blend ratios were manufactured on rotor spinning machine. The quality levels of manufactured yams were tested on Premier Tester 7000 device. As a result, comparisons between yams were done by means of taking into account the yam quality characteristics such as CV%, the number of thin-thick places, and nep. In order to understand the effect of blend ratio on yam quality with a statistical approach a one way ANOVA was performed on test results. For this aim, the statistical software package SPSS 8.0 was used to interpret the experimental data. All test results were assessed at significance levels of alpha <= 0,05 and alpha <= 0,01.