Optimization of polymerization parameters for the sorption of oseltamivir onto molecularly imprinted polymers

被引：8

作者：

Yang, Yajun ^{[1
]}

Li, Jianyong ^{[1
]}

Liu, Yurong ^{[1
]}

Zhang, Jiyu ^{[1
]}

Li, Bing ^{[1
]}

Cai, Xuepeng ^{[2
]}

机构：

[1] Chinese Acad Agr Sci, Key Lab Vet Pharmaceut Discovery, Key Lab New Anim Drug Project, Lanzhou Inst Anim Sci & Vet Pharmaceut,Minist Agr, Lanzhou 730050, Peoples R China

[2] Chinese Acad Agr Sci, Lanzhou Inst Vet Sci, Lanzhou 730000, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2011年 / 400卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Molecularly imprinted polymer (MIP); Polymerization optimization; Selectivity; Uniform design; Oseltamivir; LC-MS/MS; ANTIINFLUENZA DRUG; BINDING; ACID; PERFORMANCE; SELECTIVITY; CHROMATOGRAPHY; RECOGNITION; VALIDATION; METABOLITE; TAMIFLU(R);

D O I：

10.1007/s00216-011-5063-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecularly imprinted polymers (MIPs) are tailor-made polymers with high selectivity for a given analyte, or group of structurally related compounds. The influence of the process parameters (the moles of functional monomer and cross-linker, the selection of functional monomer and solvent) on the preparation of oseltamivir (OS)-imprinted polymers was investigated. A mathematical method for uniform design to optimize these selected parameters and to increase the MIP selectivity for template molecules was applied. The optimal conditions to synthesize MIP were 0.69 mmol 30% acrylamide (AA) + 70% 4-Vinylpyridine (4-VP) and 5.0 mmol ethylene glycol dimethacrylate (EGDMA) copolymerized in 5 ml toluene in the presence of 0.1 mmol OS. MIP showed high affinity and selectivity for separation of the template molecule from other compounds. In the present study, we have established an effective LC-MS/MS method to identify and quantify OS with good sensitivity, accuracy and precision.

引用

页码：3665 / 3674

页数：10