Anti-inflammatory activity of Chrysanthemum zawadskii var. latilobum leaf extract through haem oxygenase-1 induction

被引：27

作者：

Kim, Younghwa ^{[1
]}

Han, Jiyoung ^{[2
]}

Sung, Jeehye ^{[1
]}

Sung, Misun ^{[1
]}

Choi, Youngmin ^{[1
]}

Jeong, Heon-Sang ^{[1
]}

Lee, Junsoo ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Dept Food Sci & Technol, Cheongju 361763, Chungbuk, South Korea

[2] Doosan Corp Glonet BG, Suwon 443270, Gyeonggi, South Korea

来源：

JOURNAL OF FUNCTIONAL FOODS | 2012年 / 4卷 / 02期

关键词：

Chrysanthemum zawadskii; Anti-inflammation; Haem oxygenase-1; Nitric oxide; NF-KAPPA-B; NITRIC-OXIDE SYNTHASE; CARBON-MONOXIDE; EXPRESSION; INHIBITION; CELLS; LINARIN; PROTEIN;

D O I：

10.1016/j.jff.2012.02.007

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Chrysanthemum zawadskii var. latilobum (CZ) has been widely used as a tea in Korea. In this study, we investigated the involvement of anti-inflammatory haem oxygenase-1 (HO-1) in the inhibitory activity of a CZ leaf extracts on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. At the highest concentration of CZ leaf extract (50 mu g/mL), NO production was 10% of that in LPS-stimulated macrophages, whereas iNOS protein expression was 5% of that in LPS-stimulated macrophages. CZ leaf extract also inhibited iNOS mRNA expression in a concentration-dependant manner, and at 50 mu g/mL, iNOS mRNA expression was approximately 15% of that in LPS-stimulated cells. The CZ leaf extract induced HO-1 expression in a dose-dependent manner, and blocking HO-1 activity abolished the anti-inflammatory effect of CZ leaf extract. These results suggest that CZ leaf extract has a potent anti-inflammatory activity in RAW264.7 cells through the induction of HO-1. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：474 / 479

页数：6

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