Activatable and Cell-Penetrable Multiplex FRET Nanosensor for Profiling MT1-MMP Activity in Single Cancer Cells

被引：43

作者：

Chung, Eddie Y. ^{[1
,2
]}

Ochs, Christopher J. ^{[3
]}

Wang, Yi ^{[4
]}

Lei, Lei ^{[1
,2
,4
]}

Qin, Qin ^{[1
,2
]}

Smith, Andrew M. ^{[4
]}

Strongin, Alex Y. ^{[5
]}

Kamm, Roger ^{[3
]}

Qi, Ying-Xin ^{[6
]}

Lu, Shaoying ^{[1
,2
,4
]}

Wang, Yingxiao ^{[1
,2
,4
]}

机构：

[1] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Inst Engn Med, La Jolla, CA 92093 USA

[3] Singapore MIT Alliance Res & Technol, BioSyst & Micromech, Singapore 138602, Singapore

[4] Univ Illinois, Dept Bioengn, Champaign, IL 61801 USA

[5] Sanford Burnham Med Res Inst, La Jolla, CA 92037 USA

[6] Shanghai Jiao Tong Univ, Inst Mechanobiol & Med Engn, Shanghai 200240, Peoples R China

来源：

NANO LETTERS | 2015年 / 15卷 / 08期

关键词：

activatable cell-penetrating peptide; multiplex signals; FRET nanosensor; MT1-MMP; single cell; cancer; TYPE-1; MATRIX-METALLOPROTEINASE; QUANTUM DOTS; IN-VIVO; INTEGRIN ALPHA-V-BETA-3; PROTEOLYTIC ACTIVITY; INVASION PROGRAMS; INHIBITION; PEPTIDES; DELIVERY; MICE;

D O I：

10.1021/acs.nanolett.5b01047

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We developed a quantum-dot-based fluorescence resonance energy transfer (QD-FRET) nanosensor to visualize the activity of matrix metalloproteinase (MT1-MMP) at cell membrane. A bended peptide with multiple motifs was engineered to position the FRET pair at a close proximity to allow energy transfer, which can be cleaved by active MT1-MMP to result in FRET changes and the exposure of cell penetrating sequence. Via FRET and penetrated QD signals, the nanosensor can profile cancer cells.

引用

页码：5025 / 5032

页数：8

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