Quantitative detection of gold nanoparticles on individual, unstained cancer cells by scanning electron microscopy

被引：9
作者：
Hartsuiker, L. [1 ]
Van Es, P. [1 ]
Petersen, W. [1 ]
Van Leeuwen, T. G. [1 ,2 ]
Terstappen, L. W. M. M. [1 ]
Otto, C. [1 ]
机构：
[1] Univ Twente, MIRA Inst Biomed Technol & Tech Med, NL-7500 AE Enschede, Netherlands
[2] Univ Amsterdam, Acad Med Ctr, NL-1012 WX Amsterdam, Netherlands
来源：
JOURNAL OF MICROSCOPY | 2011年 / 244卷 / 02期
关键词：
Cancer cells; gold nanoparticles; scanning electron microscopy; NANORODS; GROWTH;
D O I：
10.1111/j.1365-2818.2011.03528.x
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Gold nanoparticles are rapidly emerging for use in biomedical applications. Characterization of the interaction and delivery of nanoparticles to cells through microscopy is important. Scanning electron microscopes have the intrinsic resolution to visualize gold nanoparticles on cells. A novel sample preparation protocol was developed to enable imaging of cells and gold nanoparticles with a conventional below lens scanning electron microscopes. The negative influence of 'charging' on the quality of scanning electron microscopes' images could be limited by deposition of biological cells on a conductive (gold) surface. The novel protocol enabled high-resolution scanning electron microscopes' imaging of small clusters and individual gold nanoparticles on uncoated cell surfaces. Gold nanoparticles could be counted on cancer cells with automated routines.
引用
收藏
页码：187 / 193
页数：7
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