Quantitative analysis of cell-free DNA in the plasma of gastric cancer patients

被引：77

作者：

Park, Jong-Lyul ^{[1
,2
]}

Kim, Hyun Ja ^{[3
]}

Choi, Bo Youl ^{[3
]}

Lee, Han-Chul ^{[1
]}

Jang, Hay-Ran ^{[1
]}

Song, Kyu Sang ^{[4
]}

Noh, Seung-Moo ^{[5
]}

Kim, Seon-Young ^{[1
,2
]}

Han, Dong Soo ^{[6
]}

Kim, Yong Sung ^{[1
,2
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, Med Genom Res Ctr, Taejon 305806, South Korea

[2] Univ Sci & Technol, Dept Funct Genom, Taejon 305806, South Korea

[3] Hanyang Univ, Coll Med, Dept Prevent Med, Seoul 133791, South Korea

[4] Chungnam Natl Univ, Dept Pathol, Taejon 301747, South Korea

[5] Chungnam Natl Univ, Dept Gen Surg, Taejon 301747, South Korea

[6] Hanyang Univ, Guri Hosp, Dept Internal Med, Gyeonggi Do 471701, South Korea

来源：

ONCOLOGY LETTERS | 2012年 / 3卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

gastric cancer; cell-free DNA; serum; plasma; Alu sequence; FREE CIRCULATING DNA; LUNG-CANCER; PROSTATE-CANCER; ALLELIC IMBALANCE; DIAGNOSTIC MARKER; TUMOR DNA; SERUM; BLOOD; QUANTIFICATION; INFLAMMATION;

D O I：

10.3892/ol.2012.592

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In the present study, an accurate and reproducible method for quantifying cell-free DNA (cfDNA) in human blood was established and tested for its ability to predict gastric cancer in patients. Using 'Alu81-qPCR' to amplify 81-bp Alu DNA sequences, we first estimated the amount of cfDNA in the serum or plasma of 130 patients with gastric cancer to identify which source of cfDNA is more suitable for the biomarker screening of these patients. The results of Alu81-qPCR revealed that the amount of cfDNA in the plasma was low compared with that in the serum, but was found at similar levels among the samples, indicating that the plasma may be a more suitable source of cfDNA for biomarker screening. For the 54 patients with gastric cancer and the 59 age-matched healthy controls, the mean levels of plasma cfDNA were 2.4-fold higher in the patient group compared with the control group, indicating that plasma cfDNA levels may be useful for predicting patients with gastric cancer. The results of our study suggest that Alu81-qPCR is a more reliable method than other techniques, such as the PicoGreen assay, for quantifying cfDNA in human blood, demonstrating the potential to complement current diagnostic procedures for the management of gastric cancer patients.

引用

页码：921 / 926

页数：6

共 31 条

[1] Role of cell-free plasma DNA as a diagnostic marker for prostate cancer [J].