A CCD-Based Reader Combined Quantum Dots-Labeled Lateral Flow Strips for Ultrasensitive Quantitative Detection of Anti-HBs Antibody

被引：55

作者：

Zhang, Xueqing ^{[1
]}

Li, Ding ^{[2
]}

Wang, Can ^{[3
]}

Zhi, Xiao ^{[1
]}

Zhang, Chunlei ^{[1
]}

Wang, Kan ^{[1
]}

Cui, Daxiang ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Dept Bionano Sci & Engn, Key Lab Thin Film & Microfabricat Technol,Res Ins, Minist Educ,Natl Key Lab Micro Nano Fabricat Tech, Shanghai 200240, Peoples R China

[2] 261 Hosp PLA, Ctr Biol Diag & Therapy, Beijing 100094, Peoples R China

[3] Cent So Univ Chan Shan, Dept Surg, Changsha 410083, Hunan, Peoples R China

来源：

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | 2012年 / 8卷 / 03期

基金：

中国国家自然科学基金;

关键词：

CCD; Lateral Flow Chip; Quantum Dots; HBV; Surface Antigen; Antibody; Quantitative Analysis; INSPECTION; SYSTEM; CELLS; DNA;

D O I：

10.1166/jbn.2012.1401

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Herein we reported a CCD-based reader combined quantum dots-labeled lateral flow strips for ultrasensitive quantitative detection of anti-HBs antibody. The CdTe quantum dots were prepared, then were used to label Hepatitis B Virus surface antigen, and then were fabricated into lateral flow strips. The as-prepared lateral flow strips were used to test different concentration of anti-HBV surface antibodies. The CCD-based reader was designed and fabricated, the quantitative analysis software was compiled, and resultant CCD-based reader system was used for quantitative analysis of examined anti-HBs antibodies on the strips. Results showed that the quantum dots-labeled lateral flow strips could detect the anti-HBs antibody with the limitation concentration of 200 pg/mL, the CCD-based reader system could detect anti-HBs antibody with the sensitivity of 2 pg/mL. In conclusion, the prepared CCD-based reader combined quantum dots-labeled lateral flow strips can be used for quantitative detection of anti-HBs antibody in sera with the sensitivity of 2 pg/mL, and has great potential in applications such as ultrasensitive detection of HBV antigens or antibodies, and other tumor biomarkers in near future.

引用

页码：372 / 379

页数：8

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