Triazole-Linked DNA as a Primer Surrogate in the Synthesis of First-Strand cDNA

被引：14

作者：

Fujino, Tomoko ^{[1
]}

Yasumoto, Ken-ichi ^{[2
]}

Yamazaki, Naomi ^{[1
]}

Hasome, Ai ^{[1
]}

Sogawa, Kazuhiro ^{[2
]}

Isobe, Hiroyuki ^{[1
]}

机构：

[1] Tohoku Univ, Dept Chem, Aoba Ku, Sendai, Miyagi 9808578, Japan

[2] Tohoku Univ, Dept Biomol Sci, Aoba Ku, Sendai, Miyagi 9808578, Japan

来源：

CHEMISTRY-AN ASIAN JOURNAL | 2011年 / 6卷 / 11期

关键词：

DNA; enzymes; oligonucleotides; primers; reverse transcription; REVERSE TRANSCRIPTION; TERMINAL ALKYNES; TEMPLATE-PRIMER; NUCLEIC-ACID; RT-PCR; (TL)DNA; EXPRESSION; OLIGOMERS; ANTISENSE; ANALOGS;

D O I：

10.1002/asia.201100712

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A phosphate-eliminated nonnatural oligonucleotide serves as a primer surrogate in reverse transcription reaction of mRNA. Despite of the nonnatural triazole linkages in the surrogate, the reverse transcriptase effectively elongated cDNA sequences on the 3'-downstream of the primer by transcription of the complementary sequence of mRNA. A structure-activity comparison with the reference natural oligonucleotides shows the superior priming activity of the surrogate containing triazole-linkages. The nonnatural linkages also protect the transcribed cDNA from digestion reactions with 5'-exonuclease and enable us to remove noise transcripts of unknown origins.

引用

页码：2956 / 2960

页数：5

共 39 条

[1] Sense or antisense? False priming reverse transcription controls are required for determining sequence orientation by reverse transcription-PCR [J].