Mechanical strain induces Cbfa1 and type X collagen expression in mandibular condyle

被引:0
作者
Van Lam, S [1 ]
Rabie, ABM [1 ]
机构
[1] Univ Hong Kong, Prince Philip Dent Hosp, Fac Dent, Biomed & Tissue Engn Res Grp & Orthodont, Hong Kong, Hong Kong, Peoples R China
来源
FRONTIERS IN BIOSCIENCE-LANDMARK | 2005年 / 10卷
关键词
Cbfa1; collagen; type X; mechanical strain;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Core binding factor a1 (Cbfa1) is a crucial transcriptional factor for chondrocyte maturation and osteoblast differentiation in the mandibular condyle. To quantitatively assess the amount of mRNA expression of Cbfa1 and type X collagen in response to mandibular advancement. 420, 35-day-old female Sprague-Dawley rats, were randomly divided into 20 experimental and 10 control groups corresponding to 10 time points. Experimental animals were advanced in either single or stepwise manner. Condylar cartilage was dissected under microscope and total RNA was extracted, Cbfa1 and type X collagen mRNA was quantified with real-time RT-PCR. Cbfa1 and type X collagen mRNA expression for all groups reached their peak on experimental day 21. During single advancement, Cbfa1 and type X collagen mRNA expression was consistently higher (3-fold and 2.8-fold respectively) than that of stepwise advancement (2-fold). The second advancement in the stepwise group resulted in both Cbfa1 and type X collagen level reaching another peak on day 51. Mandibular advancement promoted chondrocytes maturation and osteoblast differentiation by upregulating Cbfa1 expression. Stepwise advancement produced a higher level of Cbfa1 and type X collagen expression leading to bigger cartilage template onto which bone will form through endochondral ossification.
引用
收藏
页码:2966 / 2971
页数:6
相关论文
共 50 条
[31]   Stimulation with type I collagen induces changes in gene expression in peripheral blood mononuclear cells from patients with diffuse cutaneous systemic sclerosis (scleroderma) [J].
Atamas, S. P. ;
Luzina, I. G. ;
Ingels, J. ;
Choi, J. ;
Wong, W. K. ;
Furst, D. E. ;
Clements, P. J. ;
Postlethwaite, A. E. .
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2010, 161 (03) :426-435
[32]   Improving the catalytic activity for hydrogen evolution of monolayered SnSe2(1-x)S2x by mechanical strain [J].
Dong, Sha ;
Wang, Zhiguo .
BEILSTEIN JOURNAL OF NANOTECHNOLOGY, 2018, 9 :1820-1827
[33]   Troglitazone suppresses transforming growth factor-β1-induced collagen type I expression in keloid fibroblasts [J].
Zhang, G-Y. ;
Yi, C-G. ;
Li, X. ;
Ma, B. ;
Li, Z-J. ;
Chen, X-L. ;
Guo, S-Z. ;
Gao, W-Y. .
BRITISH JOURNAL OF DERMATOLOGY, 2009, 160 (04) :762-770
[34]   Accelerated degradation of collagen membranes in type 1 diabetic rats is associated with increased expression and production of several inflammatory molecules [J].
Zoabi, Hasan ;
Nemcovsky, Carlos E. ;
Bender, Omer ;
Moses, Ofer ;
Weinreb, Miron .
JOURNAL OF PERIODONTOLOGY, 2020, 91 (10) :1348-1356
[35]   Effects of human neutrophil peptide-1 on the expression of interstitial collagenase and type I collagen in human dermal fibroblasts [J].
Oono, T ;
Shirafuji, Y ;
Huh, WK ;
Akiyama, H ;
Iwatsuki, K .
ARCHIVES OF DERMATOLOGICAL RESEARCH, 2002, 294 (04) :185-189
[36]   Beneficial regulation of type I collagen and matrixmetalloproteinase-1 expression by estrogen, progesterone, and its combination in skin fibroblasts. [J].
Neena Philips ;
Jan Devaney .
Journal of the American Aging Association, 2003, 26 :59-62
[37]   Simvastatin and atorvastatin enhance gene expression of collagen type 1 and osteocalcin in primary human osteoblasts and MG-63 cultures [J].
Ruiz-Gaspa, Silvia ;
Nogues, Xavier ;
Enjuanes, Anna ;
Monllau, Joan C. ;
Blanch, Josep ;
Carreras, Ramon ;
Mellibovsky, Leonardo ;
Grinberg, Daniel ;
Balcells, Susana ;
Diez-Perez, Adolfo ;
Pedro-Botet, Juan .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2007, 101 (06) :1430-1438
[38]   Anoxia-reoxygenation stimulates collagen type-1 and MMP-1 expression in cardiac fibroblasts -: Modulation by the PPAR-γ ligand pioglitozone [J].
Chen, K ;
Li, DY ;
Zhang, XJ ;
Hermonat, PL ;
Mehta, JL .
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 2004, 44 (06) :682-687
[39]   Inhibitory effects of the transcription factor Ets-1 on the expression of type I collagen in TGF-β1-stimulated renal epithelial cells [J].
Okano, Kazuhiro ;
Hibi, Asako ;
Miyaoka, Tokiko ;
Inoue, Tomoko ;
Sugimoto, Himiko ;
Tsuchiya, Ken ;
Akiba, Takashi ;
Nitta, Kosaku .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2012, 369 (1-2) :247-254
[40]   Inhibitory effects of the transcription factor Ets-1 on the expression of type I collagen in TGF-β1-stimulated renal epithelial cells [J].
Kazuhiro Okano ;
Asako Hibi ;
Tokiko Miyaoka ;
Tomoko Inoue ;
Himiko Sugimoto ;
Ken Tsuchiya ;
Takashi Akiba ;
Kosaku Nitta .
Molecular and Cellular Biochemistry, 2012, 369 :247-254